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Microbiology > practicum > Flashcards

practicum Flashcards

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1
Q

What is the dye used to counter-stain this?

A

safranin

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2
Q

How would you describe this specimen?

A

gram-positive, bacillus

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3
Q

What test is responsible for these results?

A

catalase test

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4
Q

Which bacteria is positive for this?

A

Streptococcus pyrogenes

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5
Q

Identify these.

A

shells of diatoms

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6
Q

What cell is this?

A

Neutrophil

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7
Q

The cell labeled “b” is a(n)

A

Neutrophil

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8
Q

what type of white blood cell is located at the upper right of this image?

A

eosinophil

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9
Q

What is the full Genus species of the following microbe?

A

pseudomonas aeruginosa

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10
Q

name of the machine that measures light obserbance

A

spectrophotometer

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11
Q

dashes = um?
10x
20x
40x
100x
4x

A

10-10um
20-5um
40-2.5um
100-1um
4-25um

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12
Q

dyes used in: negative stain.

A

nigrosin

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13
Q

negative stain used to

A

measure cell size

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14
Q

dyes used in: endospore stain (2)

A

malachite green, safranin

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15
Q

dyes used in: capsule stain
stains what color?

A

india ink - backfground, safranin capsule
red background, purple capsule,

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16
Q

dyes used in: gram stain
stains what color?

A

crystal violet, iodine, alcohol, safranin
+ purple, - pink

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17
Q

dyes used in: simple stain

A

methylene blue, nigrosin

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18
Q

blood agar results (darkest yellow to least) Hemolysis

A

1) beta hemalysis, 2) alpha hemolysis. 3) gamma hemolysis

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19
Q

MSA agar is what type of media

A

selective - 7.5% salt
differential - mannitol

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20
Q

MSA agar results (3)

A

1) orange/green + fermented
2) light orange = growth
3) red = no growth

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21
Q

what bacteria is positive for msa agar?

A

staphylococcus auerus

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22
Q

catalase is what type of media?

A

differential

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23
Q

catalase does what?
results?

A

breaks down oxiders

+ bubbles
- no bubles

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24
Q

CAMP test is done on a ____ agar
what’s a positive?

A

blood
+ has an arrow

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25
Q

bile esculin test
1) type of media
2) what does it do?
3) results

A

1) differential
2) hydrolyze esculin?
3) + = top black

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26
Q

FTM
1) what does it do?
2) what does it include?chemcials
3) results

A

1) checks for type of aerobic/anaerobic
2) resazurin
3) + pink , - white

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27
Q

EMB Plate
1) what does it do?
2) what does it include?chemcials
3) results

A

1) lactose fermentation?
2) eosin & methylyne blue
3) + purple/green.
- no color

28
Q

EMB Plate

results

A

+ purple/green.
- no color

29
Q

EMB Plate

what does it include?chemcials

A

eosin & methylyne blue

30
Q

Brewer’s plate/ jar
1) what does it do?
2) what does it include?chemcials
3) results

A

1_ tests for aeronic/anaerobic
2_ thioglycotate & resazurin
3) + pink. - white

31
Q

motility agar contains

A

tetrazolium

32
Q

SIM tests for

A

sulfide production, indole, tryptophan, motility,

33
Q

SIM result: black =

A

+ sulfide

34
Q

SIM result: red after kovant reagant

A

+ indole

35
Q

MR positive result means…

A

glucose fermentation leads to mix acids

36
Q

MR results

A

+ red (Ph under5)
-yellow

37
Q

VP positive result means…

A

glucose fermentation leads to acetoin

38
Q

VP results

A

+ red (gas production)
-yellow

39
Q

phenol red fermentation tests for

A

fermentation of carbs

40
Q

phenol red uses a
2) results?

A

durham tube
2) + yellow - red

41
Q

1) phenylaline deaminase agar slant uses:
2) what color when positive

A

ferric chloride 10% , green +

42
Q

urea agar slant uses
2) what color when positive?

A

phenol red , pink = positive

43
Q

P&F agars are used only for

A

aerobes

44
Q

P&F agars test for

A

pyocyanin production

45
Q

P agar blue =

A

pseudomonas aeruginosa

46
Q

F agar fluorescent =

A

pseudomonas

47
Q

oxidase test .. tests for.

A

cytochrome C oxidase

48
Q

lysine Iron Agar Slant tests for /…. (3)

A

1) lysine breakdown
2) sulfide productin
3) glucose fermentation

49
Q

lysine Iron Agar Slant tests:
red @ top =

A

aerobic, expresses lysine deaminase

50
Q

lysine Iron Agar Slant tests:
purple @ bottom =

A

anaerobic, expresses lysine decarboxylase

51
Q

lysine Iron Agar Slant tests:
yellow =

A

positive for glucose fermentatin

52
Q

nitrate broth tests for

A

denitrification / nitrogen gas production

53
Q

nitrate tests results after reagent A/B (1) ; red =

A

makes nitrate to nitride ( red)

54
Q

nitrate tests results durham tube (before zinc); air in tube =

A

nitrate to nitrogen ( air in bubble)

55
Q

nitrate tests results durham tube (after zinc); no change =

A

positive for denitrification

56
Q

What organism is this.. causes?

A

trypanosoma. ; african sleeping sickness

57
Q

Which organism is this

A

Giardia

58
Q

which organism is this?

A

trichomonas

59
Q

what is the name of the organism depicted below? it is a colonial green alga characterized by spherical colonies with daughter cells (black dots) embedded within

A

volvox

60
Q

Identify the black structure shown.

A

zygospore

61
Q

which organism is this?

A

Penicillium

62
Q

What are present in this specimen?

A

ascospores

63
Q

forms endospores through sporulation

A

bacillus megaterium

64
Q

produces prodogisin

A

serratia marcescans

65
Q

most resistant to antibiotics

A

pseudomonoas aeruguinosa

Microbiology (7 decks)

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