Practicals (Paper 1) Flashcards
What safety precautions should be taken when using a light microscope?
- Always start with the lowest power objective under the eyepiece
- Clip the slide securely on the stage
- Adjust the light source (mirror) so that light goes up through the slide
What does the coarse focusing wheel do on a microscope?
Used to focus on the slide
Where does the cell you are drawing need to be in relation to the slide?
In the middle
What is the fine focusing wheel used for?
To focus on the cell
When should you not use the coarse focusing wheel?
When you are using a higher powered objective as the the objective could crash into the slide
What does the slide go onto?
The stage
What do you look down on a microscope?
The eyepiece
What is the bit under the stage?
The mirror
What is the bigger wheel?
The coarse focusing wheel
What is the smaller wheel?
The fine focusing wheel
What are the 3 different parts connected to the bottom of the eye piece?
The objectives
What equipment would you need when drawing a labelled diagram of a plant cell?
- Pencil
- Ruler
- Rubber
- Unlined paper
- Light microscope
What is the method for drawing a labelled diagram of a cell?
- Focus the microscope on a single cell
- Draw details of the parts of the cell that are important to your study
- Keep looking back at the cell as you draw, draw only what you see, not what you’re meant to see
What is the method used to investigate the effect of pH on enzymes (using starch and amylase)?
- Add amylase to buffered starch solution in a test tube
- Place the test tube in a water beaker that is being heated by a bunsen burner at a constant temp
- Take samples of the mixture every 10 seconds and mix them with a fresh drop of iodine solution on a dimple tile
- Repeat until the iodine stops changing colour, record the time taken for this to happen
- Repeat the procedure at different pH values
What is the method used to identify starch?
Add a few drops of iodine solution to solid food or mix with a solution of the food the iodine/solution will turn blue/black