Practicals Flashcards
Define electrophoresis
A method of analysing molecules on the basis of charge, by mesuring their migration in an electric field
Define specrophotometry
A way of analysing molecules on the basis of their spectral properties.
Define the absorbance, A, in specrophotometry.
log10(light transmitted through blank solution/light transmitted through test solution)
What is the beer lamberr law?
A = E x c x l
A is absorbance
E is extinction coefficient
c is concentration of absorbing substance
l is the path length
What is the absorbance of a solution proportional to?
The concentration of the absorbing material within it and the distance travelled by the light through the sample.
How can lymphocytes be identified with a microscope?
They are small cells with a spherical nucleus and little cytoplasm.
How can eosinophils be identified with a microscope?
Their cytoplasmic granules are bright pink, with a bi-lobed nucleus.
How may neutrophils be identified on a microscope?
They have a multilobed nucleus (polymorphonuclear) with cytoplasmic granules.
How can basophils be identified with a microscope?
Their cytoplasmic granules stain strongly blue. They have a lobed nucleus.
How can monocytes be identified with a microscope?
They have an indented nucleus with pale granules in the cytoplasm.
What is Vmax?
The maximum enzyme velocity which is allroached as substrate concentration increses.
What is Km?
Michaelis constant - the substrate concentration at which the rate of reaction is exactly half Vmax.
What is on the axes of a lineweaver-burk plot?
The x axis has 1/substrate concentration, and the y reaction 1/velocity (Vo).
What is the x intercept of a lineweaver-burk plot?
-1/Km
What is the y intercept of a lineweaver-burk plot?
1/Vmax
