Practical Zebrafish AI generated Flashcards
Describe the aim of the experiment involving zebrafish and an anti-nutritional factor.
To document changes and behavior of the neutrophils of the zebrafish when exposed to an anti-nutritional factor.
What is oral tolerance in the context of the immune system?
The process by which the immune system does not react to harmless food and microbes, while still responding to pathogens.
Define DCs in the context of the experiment.
Dendritic cells that scan the intestinal barrier, phagocytize, sample the lumen, present antigens to T-cells, and promote tolerance.
How do innate immune system components respond to damage to intestinal cells in the experiment?
Neutrophils and macrophages are recruited to the site of damage.
What is the role of saponin in the experiment?
Saponin is an anti-nutritional factor present in soy that can disrupt intestinal epithelial cell membranes.
Describe the process of observing neutrophil behavior in the experiment.
Neutrophil behavior is observed at baseline, after 1 day, and after 3 days using Image J to assess the intensity of green pixels.
What is the significance of measuring green pixels in the experiment?
Green pixels serve as a measure for GFP+ neutrophils, helping assess the impact of the anti-nutritional factor on neutrophil behavior.
Describe the in vivo model established by the authors in this paper.
The authors established an in vivo model using zebrafish for genetic analysis of the inflammatory response.
Why are the neutrophils in zebrafish larvae green?
Neutrophils in zebrafish larvae appear green because they capture green fluorescent proteins at the site of injury.
Explain the difference in neutrophil presence between control exposed and saponin exposed larvae.
There are more neutrophils in saponin-exposed larvae due to saponin activating the immune system and thus neutrophils.
What is the difference observed in neutrophil presence between different time points analyzed?
After 72 hours, more neutrophils are present in saponin-exposed larvae compared to control larvae.
How would you reflect more on what is happening in the gut when analyzing the pictures differently?
To reflect more on the gut, one could select only the gut area for analysis in a similar experiment.
What does it indicate when measuring more fluorescent pixels in the images? Is this measurement considered accurate?
More fluorescent pixels indicate more activated neutrophils. This measurement is considered accurate as it measures GFP+ neutrophils.
Describe the importance measuring the intensity of fluorescence in a study.
Measuring the intensity of fluorescence is crucial as it accounts for in the number of cells represented by the same pixel, providing a more accurate assessment.
How can future studies improve the measurement accuracy in fluorescence analysis?
Future studies can enhance measurement accuracy by using a more precise method that yields consistent results upon repetition.
