Practical 3 Flashcards
1
Q
define: anti sense strand
A
template strand, non coding (used to build new strand)
2
Q
define: sense strand
A
same as coding strand (non template) but without Uracil
3
Q
transcription produces?
A
pre-mRNA
4
Q
what is added to ends of pre-mRNA:
A
poly A tail 3’
5’ CAP
5
Q
what is removed to form mature mRNA:
A
introns are removed
6
Q
how many possible reading frames:
A
3, 1 codon has 3 triplets
- need gene orientation from promotor
7
Q
what happens to go from polypeptide to protein?
A
polypeptide: amino acid string
- other modifications are added (eg. lipids)
- folding into 3D structure forms functional protein
- also parts which are hydrophobic, hydrophilic and charged will affect shape
8
Q
DNA template strand runs from which end?
A
3’ to 5’
