Practical #1 How Small is it Anyway

Question 1

How do you calculate field of view?

Answer 1

(FOV Original)(Magnification Original)=(FOV Final)(Magnification Final)

Question 2

Example: Calculate the final FOV using the 40x objective with an original FOV of 4mm

Answer 2

(4mm)(4x)=(FOV Final)(40)

16=(FOV Final)(40)
40x 40x

(16/40x)= 0.4 mm

Question 3

How do calculate actual size? If you see 15 cells across the FOV

Answer 3

(0.4/15)= 0.026mm

Question 4

Metric System

Answer 4

1 Inch————-2.54cm
1 cm—————10mm
1mm————-1,000 Micrometers
1 Micrometer—————1,000 Nanometers