PP 1 Cell Injury And Death Flashcards
2 causes of cell injury
Environmental
Non environmental
Environment cause of cell injury
Hypoxia
Toxins/poisons
Immune mediated
Physical agents
Infection
Nutritional/dietary
Non environmental causes of cell injury
Genetics
Ageing
Hypoxia meaning
Oxygen deprivation
Causes of hypoxia
Hypoxaemic hypoxia
Anaemic hypoxia
Ischaemic hypoxia
Histotoxic hypoxia
What is hypoxaemic hypoxia?
Arterial content of O2 is low
What is anaemia hypoxia?
Example
Decreased ability of haemoglobin to carry oxygen
e.g. carbon monoxide poisoning
What is ischaemic hypoxia?
Interruption of blood supply
What is histotoxic hypoxia?
Inability to utilise O2 due to disabled oxidative phosphorylation enzyme
How long does it take for neurones to be affected by hypoxia?
A few minutes
why is CPR needed ASAP in cardiac arrest?
Neurones are affected by hypoxia after a few minutes
To prevent brain damage
How long does it take for skeletal muscle to be affected by hypoxia?
A few hours
Examples of toxins
Poison
Pollutant
Insecticides
Herbicides
Asbestos
Alcohol
Drugs
What is a hypersensitivity reaction?
Example
Injury secondary to excessive immune reaction to a non self antigen
E.g. anaphylaxis
Physical agents examples
Trauma
Extreme temp. - frost bite, burns
Electric currents
Radiotherapy
Types of microbes
Bacterial
Viral
Parasitic
Fungal
Nutritional/dietary issues which can cause cell injury
Obesity
Anorexia
Dietary deficiency or excesses - B12/folate/vit D/fat
Issues due to genetics/ageing
Inborn errors of metabolism
Enzyme deficiencies
Dysfunctional proteins
What happens in reversible cell damage?
- swelling: due to Na+/K pump failure
- cytoplasmic blebs
- clumped chromatin: due to reduced pH
- ribosome dispersion: lack of ATP to hold together
- membrane remains intact
What happens in irreversible cell damage?
- nuclear changes
- membrane defects > causes lysis of ER
- lysosome rupture
What is cell death due to?
Irreversible cell injury
Types of cell death
Apoptosis
Necrosis
Apoptosis meaning
Individual programmed cell death
Describe apoptosis
Single cell death
Cell shrinks
Plasma membrane preserved
Organelles contract
DNA cleaved between nucleosides
Dead cells taken up by phagocytosis
Pathways of apoptosis
Intrinsic (mitochondrial)
Extrinsic (death receptor)
Intrinsic pathway of apoptosis
Mitochondria released cytochrome C
Activates caspases which induce apoptosis
What are caspases?
Enzymes involved in cell death
Extrinsic pathway of apoptosis
Death receptors attach to cell membrane which activates caspases > apoptosis
What are death receptors secreted by?
T killer cells
Describe necrosis
Grouped cell death
Cell swells
Plasma membrane destroyed
Organelles swell and break down
DNA is degraded randomly
Dead cells start inflammatory process
Characteristic nuclear changes in necrosis
Pyknosis
Karyorrhexis
Karyolysis
What is pyknosis?
Nuclear shrinkage
What is karyorrhexis?
Nuclear fragmentation
What is karyolysis?
Nuclear dissolution