Postlab - Carbo (TLC)

Question 1

TLC separate products in the _____ (glucose) and ____ (glucose, maltose and dextrin) hydrolysates of starch and glycogen

Answer 1

Acid
Enzymatic

Question 2

What is under acid?

Answer 2

Glucose

Question 3

What is under enzymatic?

Answer 3

GLucose
Maltose
Dextrin

Question 4

What is in the staionary phasE?

Answer 4

Silica gel

Question 5

what is in the mobile phasE?

Answer 5

Solvent systems used

Question 6

Is it true that Staionary phase (silica gel) is more polar than the mobile phase (solvent systems used)

Answer 6

Yes true

Question 7

What silica gel used in the experiment?

Answer 7

Normal phase — Silica gel G

Question 8

Silica gel G
What does G means?

Answer 8

Gypsum (CaSO4)

Question 9

Function of Silica gel G Gypsum

Answer 9

Binds silica gel on one side of metal shet (inert support)

Question 10

What does sthe surface of the gel contains? What functional group?

Answer 10

-OH groups

Question 11

What physical interaction may involved in Normal Phase Silica gel?

Answer 11

Hydrogen bonding or Dipole-dipole (polar) interaction network of silicic acid (orthosilicic acid), ; polysilicic acid (polymer)

Question 12

Direction of solvent front

Ascending or Descending

Answer 12

Ascending

Question 13

Principle involved in separation based on physical states of the stationary and mobile phases:

Answer 13

Adsorption

Question 14

Separation of the carbohydrates in the hydrolysate will depend on their _______.

Answer 14

polarity

Question 15

What is its Solvent System, what’s the ratio Acetonitrile:Water

Answer 15

85:15

Question 16

What is the percentage of 1- napthyl EDTA

Answer 16

0.3%

Question 17

What is the percentage of Sulfuric acid in MetOH?

Answer 17

5%

Question 18

What is the visualizing agent?

Answer 18

p-anisaldehyde

Question 19

What are the 3 colored spots?

Answer 19

Blue/Blue-green/Green

Question 20

The more –OH groups, the more polar the sugar is

True / Flase?

Answer 20

True

Question 21

glucose > maltose > dextrin
(dextrin stayed on the origin)

Interpret

Answer 21

glucose is simpler than maltose, and maltose is simpler than dextrin in terms of their molecular structures.