Postlab - Carbo (Quantitative Analysis)

Question 1

The amount of carbohydrates present in a given sample can be measured by what method?

Answer 1

Nelson’s Methods

Question 2

Nelsons’ mthods is based on the capacity of the free reducing groups of sugars in a carbohydrate sample to reduce Cu⁺² in what solution?

Answer 2

Alkaline solution

Question 3

Is it true that Nelson’s Methods is sensitive and reproducible

Answer 3

Yes true

Question 4

The amount of reducing sugars in the sample is measured ______.

Answer 4

Colorimetrically

Question 5

The amount of sugars are directly related to the ______ ______ formed via series of redoz reactions

Answer 5

Molybdenum blu

Question 6

Nelson’s A or B:

Rochelle salt – sodium potassium tartrate, Na₂CO₃, NaHCO₃, Na₂SO₄

Answer 6

Nelson’s A

Question 7

Nelson’s A or B:

CuSO₄·5H₂O, H₂SO₄

Answer 7

Nelson’s B

Question 8

What reagent used in this:

(NH₄)₂MoO₄, H₂SO₄, Na₂HAsO₄ ∙ 7H₂O

Answer 8

Arsenomolybdate reagent

Question 9

Colorimetry of Absorbance is based on the intensity of the product of _________ and the _______ solution

blue / blue-green solution (A₄₈₀)

Answer 9

arsenomolybdate
copper oxide solution

Question 10

t/F:

the intensity of the blue coloration is dependent on the amount of Cu₂O formed w/c is also related to the amount of reducing sugar oxidized

Answer 10

True