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3S - Biochem Lab > Postlab - Carbo (Qualitative Tests) > Flashcards

Postlab - Carbo (Qualitative Tests) Flashcards

1
Q

Test for reducing sugars

A

Benedict’s Test

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2
Q

Test that is sensitive than Fehling’s Test

A

Benedict’s Test

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3
Q

Reagents used in Benedict’s Test

A

CuSO₄
Na₂CO₃
Na₃C₆H₅O₇

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4
Q

Test where:

Cu²⁺ oxidizing agent (OXIDATION)

A

Benedeict’s test
Barfoed’s Test

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5
Q

Test where principle is:

Cupric ions reduced to cuprous, form Cu₂O precipitate

A

Benedict’s Test
Barfoed’s Test

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6
Q

Test where Principle is:

Sodium citrate (BASIC) used to keep Cu ions in solution

A

Benedict’s Test

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7
Q

What color of the precipitate for all reducing sacchardies in benedict’s test

A

Brick-Red

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8
Q

What test has the “Brick-red” precipitate for all reducing saccharides

A

Benedict’s Test

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9
Q

Test that refers to:

All monosaccharides are reducing saccharides

A

Benedict’s test

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10
Q

What test is this?

Glucose ——Blue——> Gluconic Acid

A

Benedict’s test

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11
Q

WHat test distinguishes reducing monosaccharides & disaccharides
(mono- forms Cu₂O faster than di- in acidic medium)

A

Barfoed’s test

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12
Q

Reagents used are Cu(CH₃COO)₂, acetic acid. What test is it?

A

Barfoed’s Test

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13
Q

Test result of Barfoed’s Test in monosaccharide

A

Brick-red in less than 3 minutes

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14
Q

Test result of Barfoed’s Test in disaccharide

A

Brick-red for around 10 minutes
(considered as a negative result)

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15
Q

What test uses these reagents

HCl, FeCl₃ and orcinol

A

Bial’s Orcinol Test

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16
Q

What test involved this principle?

Strong acid to dehydrate monosaccharide
to produce furfural or hydroxymethylfurfural

A

Bial’s Orcinol Test

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17
Q

What test will then condense with orcino?

A

Bial’s Orcinol Test

18
Q

Visual Positive result of Bbial’s Orcinol Test

A

Blue-green solution for aldopentoses

19
Q

Test specfici for galactose and lactose

A

Mucic Acid Test

20
Q

True of False
In Mucic Acid Test:
- formyl functional group will get oxidized to carboxylic acid
- Primary alcohol group will get oxidized to carboxylic acid

A

True

21
Q

What test is this associated?

BROKEN GLASS- LIKE/RHOMBIC CRYSTALS

A

Mucic Acid Test

22
Q

Mucic Acid Test is Plane of symmetry – not optically active

T or F

A

True

23
Q

Test for all sugars w/ free carbonyl group at C1 or C2

A

Phenylhydrazone or Osazone Test

24
Q

Test where reagent used is phenylhydrazine

A

Phenylhydrazone/Osozone Test

25
Q

Test where principle involved is:
- phenylhydrazine is a strong oxidizing agent

A

Phenylhydrazone/osozone Test

26
Q

In Phenylhdrazone test, formyl functional group will get oxidized to carboxylic acid.

T/F

A

True

27
Q

T/F:

In Phenylhydrazone/Osozone Test, Secondary alcohol will get oxidized to ketone

A

True

28
Q

Positive result of Phenylhydrazone

A

Yellow orange crystals (osazones)
D-glucosazone (for glucose)
“No sucrosazone”

29
Q

Appearance of Glucose and Fructose

A

Broom stick/needle shaped/feather like crystals of glucosazone and fructosazone

30
Q

Broom stick/needle shaped/feather like crystals of glucosazone and fructosazone

A

Glucose
Fructose

31
Q

Sunflower shaped crystals of matosazone

A

Mannnose

32
Q

Appearance of Mannose

A

Sunflower shaped crystals of maltosazone

33
Q

Hedgehog or cotton ball-shaped crystals of lactosazone

A

Lactose

34
Q

Appearnce of Lactose

A

Hedgehog or cotton ball-shaped crystals of lactosazone

35
Q

Phenylhydrazone/Osazone Test:

Sugar: Mannose
Time: ?
Charac: Needl-like/Feathery
Name: GLucosazone

A

0.5 minutes

36
Q

Phenylhydrazone/Osazone Test:

Sugar: Fructose
Time: ?
Charac: Needl-like/Feathery
Name: GLucosazone

A

2 minutes

37
Q

Phenylhydrazone/Osazone Test:

Sugar: Glucoe
Time: ?
Charac: Needl-like/Feathery
Name: GLucosazone

A

4-5 minutes

38
Q

Phenylhydrazone/Osazone Test:

Sugar: Xylose
Time: ?
Charac: Long, Fine Needle-like Crystals
Name: Xylosazone

A

7 minutes

39
Q

Phenylhydrazone/Osazone Test:

Sugar: Galactose
Time: ?
Charac: Broad/Flat Crystals
Name: Galaztosazone

A

15-19 minutes

40
Q

Phenylhydrazone/Osazone Test:

Sugar: Maltose
Time: ?
Charac: Broad needle-like crystals
Name: Maltosazone

A

Soluble in how water

41
Q

Phenylhydrazone/Osazone Test:

Sugar: Lactose
Time: ?
Charac: Fine needles, groups in balls
Name: Lacctosazone

A

Soluble in hot water

3S - Biochem Lab (5 decks)

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