Postlab - Carbo (General Tests) Flashcards
Reagents used are:
H₂SO₄, EtOH and α-naphthol
Molisch’s Test
What test is involved in this principle?
Strong acid to dehydrate monosaccharide to
produce furfural or hydroxymethylfurfural
Molisch’s test
What test is involved in this principle?
Intermediate furfural derivatives will condense
with α-naphthol
Molisch’s Test
Visual + result of Molisch’s Test
Purple Ring at interphase for all carbohydrates
What is the positive results of Molisch’s Test?
Purple Ring
What color of the solution is this? Under Iodine Test
Statch —> Starch-iodo complex
Blue-Black Solution
What color of the solution is this? Under Iodine Test
Statch —> Glycogen-iodo complex
Red or Pink solution
In amylose, 6 turns of the helix equivalent to ______ how many glucose units are required to accommodate I₂ in forming the characteristic blue color ofthe complex.
36 glucose units
In amylose, how many turns of the helix equivalent to 36 glucose units are required to accommodate I₂ in forming the characteristic blue color ofthe complex.
6 turns
