PLTS (lect 4) Flashcards
PLt function
plt plug
fibrin form
vascular integrity
When are plts transfused
to increase
prophylactically
function?
to increased plt count
prophyactically for oncology
if plts arent fuctioning (PFA)
Storage
what can happen during storage?
what is released?
what decreases?
agg
plts can be activated in storage
ATP relased
pH decreases
plts aggregate
PLT storage lesion
loss of quality during storage
Plt storage
temp
continuous
important factors
expires…post collection or…if?
20-24c
continuous agitation
expires 5 days post collection, up to 7 days if you test for bacteria before use
alternative storage
what changes plasma in bag?
stored for how long?
PAS changes plasma in bag
frozen for up to 2 yrs
Pathogen inactivation
UV light to inactivate DNA/Viruses
removes pathogens
Collection requirements
viable pH
plt count
what if possible?
pH >6.2 to be viable
PLTS: >5.5 x 1010
leukoreduced possible
Donor qualifications
plt count
aspirin deferral
plavix deferral
donation time period?
plts >150,000
Aspirin 2 day deferral
plavix 14 day deferral
donate every 2 days
Apheresis
anticoag
centr
filtr
anticoag- citrate
centrifige
filtration
Granulocytes
DD
Stim with
<24hr
used for?
ABO ? Must be?
DIRECTED DONATION
stim with corticosteriods/CSF
<24hr RT
used for neutropenic pts transfusions for infections
ABO matched, must be irradiated
HPCS
growth factors
Pretransfusion testing
before admission?
not required?
what should be negative?
ABO/RH preformed before admission to hospital
NOT REQUIRED TO TO CROSS MATCH, AB SCREEN, ABO MATCH
CMV negative
HLA
dise….trans…plt
what chromosome?
highly? what happens frequently on far apart loci?
expression?
inherited via?
disease association, transplants, plt transfusions
Chrom 6
highly polymorphic, crossover frequently on far apart loci
CO-DOM expresses 2 allels for each locus
inherited via haplotype
CREG
what group?
any…
cross reactivity group
any haplotype will react with any of the abs
