Donor Selection, phlebotomy and testing (Lect 1) Flashcards

1
Q

Inventory management

A

blood is scare and renewable, less than 10% donate, volunteer donors

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2
Q

Prioritization
blood collection process

A

blood collection process

Donor Selection: regsitration, ID, education

Determine eligebility: Health history, mini physical

Collection: phlebotomy, post care

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3
Q

Selection: Donor ID

Date of last donations
WB
Double rbc
plts
plasma

A

Photo ID check >16yrs
(school ID, passport, ID..ect)

Date of last donation
WB: 56 days
Double rbcs: 112 days (3 a year)
PLTS: 7 days, (24 a year)
Plasma: 28 days (13 a year)

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4
Q

Deferral status

A

should be checked against a record of all perm deerral donors

consent to donate

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5
Q

Mini physical

allogenic H/H male vs female

Autologous

Temp
BP
Pulse
Weight

A

general appearance

Anemia:

Allogenic H/H >12.5g/dl or 38% F
>13g/dl Males

Autologous: H/H >11g/dl or >33%

Temp not over 27.5-99.5

BP: <180/100
pulse: 50-100

Weight >110lbs
MAX 10.5kg/ml

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6
Q

Eligibility Donor History Questionare DHQ
used to?
meds/vacc

i/b
b
c
c
b

A

Used to protect donor and recipient
medication lists/vaccines

Infectious diseases - blood parasites (malaria)
Bacterial viruses (zika)
Cardiac strain
coag
birth defects

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7
Q

Travel restrictions

A

malaria endemic regions deferred 1-3yrs

Bebesia year round NAT testing

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8
Q

Ruling out infectious agents

Qs only

donor test only

Qs/testing

Testing blood

Testing negative blood

A

questions only - malaria, prions, ebola

Donor test only - WNV, Chagas

Q’s/testing - HIV,HBV.HCV, zika, bebeisa, syphilis

Test blood - bacterial

Test negative blood - CMV

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9
Q

Phlebotomy

Vasovageal rxn

hyperventilation

Hypotensive shock

A

cannot exceed 15 min

Vasovagal rxn: most common primary sign is brachycarida

Hyperventilation: common 1st time donors

Hypotensive shock: uncommon, primary sign tachycardia

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10
Q

Allogenic decisions

A

genetically non related donor must adhere to strict collection standards

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11
Q

Autologous decision

A

self donation
TACO
transfusion associated circulatory overload

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11
Q

Directed decisions

A

family/friends donoate for specific recipients, mostly for rare genetic traits
low risk allo

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12
Q

Adjusting the anticoag for low volumes

A

rare donors accepted under 110llbs

63ml is std anticoag in 450ml

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13
Q

Temporary deferral

A

donor umable to donate due to current health or risk of infection

2wks to 3 yrs

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14
Q

Indefinite deferral

A

not determined when donor can be re-entered into pool

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15
Q

Permanent deferral

A

donor will never be able to donate due to test or questions

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16
Q

deferral time
Today

A

because of potential bacterial contam or donor health

17
Q

deferral time
48 hrs

A

aspirin for plts

18
Q

deferral time
2wks

A

vaccines (live attenuated/abx)

19
Q

deferral time
6wks

A

pregnancy

20
Q

deferral time
8wks

A

donation time frame

21
Q

deferral time
3 mos

A

risky behavior, STD treated, tranfusion

22
Q

deferral time
12mos

A

incarceration

23
Q

deferral time
2yrs

A

postivie bebesia NAT

24
Q

deferral time
3yrs

A

malaria infection or living in malaria area

25
Q

deferral time
indefinite/permanent

A

Pos Hep or HIV/VCJD heart or lung condition

26
Q

Testing of donor blood

A

ABO for weak blood groups
RH weak D
AB screen +ab doesnt mean no donation

Phenotype to locate rare ags

27
Q

Required viral testing

A

Syphilis VDRL
Hep B ab

HepB/C/WNV/bebsia NAT

28
Q

Required tests

A

blood type/screen
Hep B/C
WNV
syphilis
bebesia
WBC count on leukoreduced units

29
Q

Treponema pallidum (syphilis)

A

deferral 12 mos post treatment

RPR/Hemaagglutinin
FTA-ABS, EIA tests

30
Q

HBV

A

Deferral permanent

HBSAG test

Anti-HBc (antibody made with infection)

NAT TEST

31
Q

HIV

A

permanent deferral
NAT
Anti-HIV
confirm w ELISA

32
Q

WNV

A

deferral 120 days
RNA/NAT
mini-pools
1/5 people dont show symtoms

33
Q

HCV

A

deferral indefinite
Anti-HCV ELISA
NAT

34
Q

Bebesiosis

A

deferral 2 yrs from reactive test
RNA/NAT
maltese cross

35
Q

HTLV

A

deferral permanent
Anti-HTLV CHLIA
western blot

36
Q

Zika

A

deferral 4 weeks

37
Q

Chagas

A

deferral indefinite
all donors tested once

38
Q

Covid

A

14 day deferral

39
Q

Donor re-entry

A

look backs kept for 10yrs

40
Q

Live attenuated vaccines

synthetic

A

deferral for 2-4weeks

synthetic no deferral

41
Q
A