Plaque/biofilm

Question 1

4. Plaque Biofilm

Answer 1

bacterial plaque/microbial plaque

Question 2

4. Plaque Biofilm/bacterial plaque/microbial plaque

Answer 2

a. Dense, non-calcified, organized mass of cooperating colonies enclosed in a gel-like intercellular matrix
b. Formation
i. Initial formation = 2 hours
ii. 2 days to double – can visually see it at this point
c. Initial colonizers = Gram Positive Aerobic and facultative organisms (actinomyces and streptococcus)
i. Non-motile
d. Secondary colonizers = Gram Negative Anerobic
i. DO NOT colonize a clean tooth
ii. Pi and Pg, spirochetes, motile rods
iii. Etiological source of caries and perio disease
e. Timeline = pellicle 🡪 then healthy bacteria 🡪 bad bacteria 🡪 plaque maturation
f. Matrix
i. When nutrients added these products get produced:
1. Glucans (dextrans) & Fructans (levans)
g. Plaque removal
i. Plaque cannot be removed w/ water spray
ii. Anerobic glycolysis results in pH drop from 7.0 🡪 4.5 = demineralization
5. Calculus

Question 3

b. Formation

Answer 3

i. Initial formation = 2 hours
ii. 2 days to double – can visually see it at this point

Question 4

c. Initial colonizers

Answer 4

Gram Positive Aerobic and facultative organisms (actinomyces and streptococcus)
i. Non-motile

Question 5

d. Secondary colonizers

Answer 5

Gram Negative Anerobic

i. DO NOT colonize a clean tooth
ii. Pi and Pg, spirochetes, motile rods
iii. Etiological source of caries and perio disease

Question 6

e. Timeline

Answer 6

pellicle 🡪 then healthy bacteria 🡪 bad bacteria 🡪 plaque maturation

Question 7

f. Matrix

Answer 7

i. When nutrients added these products get produced:
1. Glucans (dextrans) & Fructans (levans)

Question 8

g. Plaque removal

Answer 8

i. Plaque cannot be removed w/ water spray
ii. Anerobic glycolysis results in pH drop from 7.0 🡪 4.5 = demineralization