plant breeding Flashcards
mutation examples
mutant wheat- enhanced nitrogen use efficiency
hgh yielding pest reistance soybean variety-profitability
mutatnt tomato with increased selflife
**mutant maize **- increased photosynthetic efficiency
**mutant grapevine **resistance to powdery mildew
mutant rose with unique colors
**mutant cassava ** high nutritional content
eg
1. shabarmati sonora mutant wheat- improved bread making quality
2. rio red grapefruit varirty deep flesh color
3. Golden Promise Barley:mutant barley with improved maltin quality
male striity
- GMS IN MAIZE msgenes
- CMS IN RICE wild abort typr
- CGMS IN SUNFLOWER pet1cytoplasm
- TGMS RICE-pei ai 64s-high temperatur sterile
- PGMS RICE-nongkhen58s- longday sterile
- chemically induced-wheat using ethral or sodium methyl arsenate
- transgenic-Barnase/Barstar System in Brassica napus (Canola)
ways to overcome selfincompatibility
- bud pollinaton- apples and pears
- mixed pollination- cabbage
- artificial pollination-alfalfa
- invitro pollination-orchids and fruit crops
- transgenic modifiction-tobacco successs
- chemical treatment -calcium chloride
- grafting-cherry
8.
gametophytic self incompatiblity
apple
tomato
sporophytic self incomm
sunflower
cabbage
DNA Fingerprinting
also known as DNA profiling, is a molecular technique used to identify and evaluate the genetic makeup of an individual organism by analyzing specific regions of their DNA.
dna fingerprinting
variety identification and authentification
genetic diversity verifcation and conservation programs
marker assisted selection in breeding programs
hybrid seed production
tracabiity and food safety
parental tracking
disease resistnace
Molecular markers
are fragments of DNA that are associated with a particular location within the genome and can be used to identify individuals or species, as well as to associate genetic variation with traits of interest.
soatic hybridiztaion
- potato-s.tuberosum*s.bulbocastanum-late blight resistance
- rice-oryza sativa*o.officinalis-salinity and stress tolerance
- tomato-s.lycopersicum*wild relative-increased vitamin and minerls
- citrus-seed lessness*disease resistance-improved yield
embryo rescue technique
plant tissue culture technique used to save and grow embryos from seeds that might not otherwise develop to maturity