Pipetting Technique and Centrifugation (P) Flashcards
What is pipetting technique?
A technique used to transfer aliquot of liquid sample from one container to another
What are the different types of pipettes?
1) According to Design
2) According to Drainage
3) According to Type
What are the different types of pipettes under according to design type of pipette?
1) To contain (TC)
2) To deliver (TD)
What is TC?
TC pipette holds or contains a particular volume but does not dispense that exact volume
What is TD?
TD will dispense the volume indicated
What type of pipette under according to design type is more common?
TD pipettes are more common than TC pipettes
What are the different types of pipettes under according to drainage type of pipette?
1) Blow-out
2) Self-draining
What is the characteristic of blow-out pipette?
Blow-out pipette has frosted bands or two continuous rings or etched rings at the top
What should be done when blow-out pipette is used?
The last drop is fully expelled into the receiving vessel
What should be used when expelling the last drop of liquid or reagent in blow-out pipette?
Aspirator bulb
What is self-draining pipette?
The contents of self-draining pipette are drained by gravity
What are the types of pipette under according to type pipettes?
Measuring or Graduated
What are the functions of measuring or graduated pipettes?
Used to transfer reagents and make dilutions
Used to dispense several volumes
What are the 3 types of measuring or graduated pipettes?
1) Serologic
2) Mohr
3) Micropipette
What are the characteristics of serologic pipette?
1) The graduations are located after the tip of the pipette
2) It is a blow-out pipette
What is a Mohr pipette?
1) The graduations are located before the tip of the pipette
2) It is a self-draining pipette
What is the volume capacity of micropipette?
< 1 mL