Pipet Calibration Semi Annual Flashcards
What is the pipet volume for the semi-annual calibration?
300UL
This refers to the volume capacity of the pipette being calibrated.
What is the serial number of the pipet?
A68031022
This unique identifier helps track the specific pipette for calibration records.
What is the accuracy of the balance used in the calibration?
+0.001g
This indicates the precision of the balance in measuring weight.
What is the method used for volume testing?
Gravimetric Volume Testing
This method involves weighing a liquid to determine its volume.
What is the target volume for the gravimetric volume testing?
0.300 mL
This is the volume of deionized or distilled water used for testing.
What are the individual weight limits for the pipet calibration?
+/-5%: 0.285 - 0.315 g
This range indicates acceptable weight variations during testing.
Where is the pipet located?
Receiving Bay, Room # 1
This specifies the physical location of the pipette in the lab.
What was the temperature at the time of calibration?
25°C
Temperature can affect liquid density and measurement accuracy.
What is the average weight recorded during the calibration?
0.300 g
This average weight indicates that the pipette dispenses the correct volume.
What is the pass/fail result of the calibration?
Pass: V
This indicates that the pipette calibration met the required standards.
What is the next due date for calibration?
07/02/10
This indicates when the next calibration is scheduled.
Fill in the blank: The balance serial number is _______.
1117132085
This unique identifier is essential for maintaining quality assurance.
What is a Traceable Thermometer checked at annually?
Ice Point
The thermometer is tagged with the date, identification, temperature checked, and correction information.
What is the accuracy of the Digital Thermometer?
+/- 2°F / +/- 1°C
This refers to the precision in temperature measurement.
What is the resolution of the Digital Thermometer?
0.1°F / C
This indicates the smallest change in temperature that can be displayed by the thermometer.
What is the temperature range of the 6” Alcohol Thermometer?
58° to 300°F / -50° to 150°C
This defines the minimum and maximum temperatures that can be measured.
What is the sampling time for the Digital Thermometer?
1.5 seconds
This is the time it takes for the thermometer to provide a reading.
How often should the temperature of the incubator be checked?
Daily
Regular checks ensure that the incubator maintains the proper temperature for optimal conditions.
Where can the temperature be checked on the Charm EZ?
Above the Charm EZ label
This is the designated area for monitoring temperature on the device.
Where can the temperature be checked on an external incubator?
Back of the incubator beneath the hinges
This location is specified for external incubator monitoring.
If the temperature is out of range, whom should you contact?
Charm Technical Support
This ensures that any issues are addressed promptly by qualified personnel.
Fill in the blank: The Digital Thermometer uses a _______ battery.
1.5 volt button
This specifies the type of power source used for the thermometer.
What is the maximum allowable temperature for raw milk TC samples upon receipt?
4.4° C
Samples exceeding this temperature should not be tested.
What temperature must the sample be maintained below?
7° C
It is crucial to ensure the sample temperature remains below this limit.
What should be done if a raw milk TC sample is overfilled?
Do not accept the sample
Overfilled samples can lead to inaccurate testing results.
What is required for proper mixing of the sample?
Sufficient head space
Allowing head space is important to facilitate effective mixing.
What is the first instruction regarding the test strip container?
Always reseal container with this lid immediately after opening
This ensures the integrity of the reagents.
What is the storage requirement for the sealed container?
Refrigerate
Proper refrigeration is necessary to maintain reagent stability.
What should be recorded in the reader and on the daily antibiotic log sheets?
Lot # of the test strips
This tracking is essential for quality control and traceability.
What color should the desiccant be?
Blue
Indicates that the desiccant is functioning properly.
How should the test strips be prepared for use?
Set out number of strips to be used and place them in a dry labeled container at room temperature
This preparation helps to avoid moisture contamination.
What should be done with any leftover test strips at the end of the day?
Discard any leftover
This prevents the use of expired or contaminated strips.
What is the sample preparation time for the Beta-lactam Test?
Milk sample and controls 25 times in 7 seconds
This refers to the rapid preparation required for accurate testing.
What should be done to avoid foam when preparing the sample?
Allow foam/bubbles to settle or tilt sample to avoid foam
Proper handling is crucial for accurate results.
How soon should the sample be used after shaking?
Use within 3 minutes of shaking
Timeliness is important to maintain sample integrity.
What is the time limit for pipetting the sample after placing the first strip in the incubator for SL3?
Pipet sample within 1 minute 15 seconds
This is critical for ensuring accurate test results.
What is the time limit for pipetting the sample after placing the first strip in the incubator for SLBL?
Pipet sample within 2 minutes
Adhering to this timeframe is essential for accuracy.
What should each test strip be labeled with?
Sample identification such as sample #, manifest or route #, and indicate F (Front) or R (Rear) if applicable.
Where should the test strip be placed after labeling?
In the Incubator.
What should you do before using the sample compartment of the test strip?
Peel the backing open to expose the sample compartment.
What does ‘CHARM’ refer to in the context of the document?
CHARM SCIENCES INC.
What is the proper angle to hold a pipet sample?
At an angle to avoid foam and bubbles
Holding the pipet at an angle helps to minimize the introduction of air and prevents foaming.
How should a pipet be positioned when in use?
Keep pipet vertical
A vertical position ensures accurate measurement and avoids contamination.
What is the maximum depth a pipet tip should be inserted into a sample?
No more than 1 cm
This prevents damage to the pipet and ensures consistent sample handling.
What is the maximum time allowed to complete all samples in the Charm 3 SL3 Beta-lactam Test?
1 minute 15 seconds
What is the incubation time for the Charm 3 SL3 Beta-lactam Test?
3 minutes
What is the maximum incubation time for the test strips in the Charm 3 SL3 Beta-lactam Test?
3 minutes and 30 seconds
How should the pipet be positioned when adding a sample to the strip?
Upright and vertically above the strip with the tip in either side well of the sample pad compartment
What should you avoid touching when adding the sample?
The sponge
What is the procedure after adding all samples to the strip?
Re-seal strip before starting 2nd test
What should be done after pipetting all samples?
Close latch
What starts automatically after closing the latch in the Charm 3 SL3 Beta-lactam Test?
Automatic timer
Is the automatic timer required for the Charm 3 SL3 Beta-lactam Test?
No
Fill in the blank: Use the _______ line as a guide.
Etched
What is the maximum incubation time for the Charm SLBL Beta-lactam Test?
9 minutes
The incubation should ideally be for 8 minutes.
What should be done with samples after placing the first test strip in the incubator?
Complete all samples within 2 minutes
This is crucial for maintaining test accuracy.
What is the recommended position for the pipette during the test?
Upright and vertically above strip
The tip should be in either side well of the sample pad compartment.
What should be avoided when adding the sample to the test strip?
Touching the sponge
This helps prevent contamination.
What should be done before starting the second test?
Re-seal the strip
Ensures that the test conditions remain controlled.
What action should be taken after pipetting all samples?
Close the latch
This is important to secure the test environment.
What happens when the latch is closed after pipetting?
An automatic timer will start
The timer helps to track the incubation time.
Is the automatic timer required for the Charm SLBL Beta-lactam Test?
No, it is not required
Manual timing can also be used.
What is the purpose of the etched line in the test procedure?
Use as a guide
It helps in proper positioning of the pipette.
What should be visually inspected in the beta-lactam tests?
C (control) line for full development
The control line indicates whether the test was valid or not.
What does an absent C line or partial C line indicate?
An invalid test
In such cases, the sample must be re-tested.
What is the time limit for reading strips for SL3 after incubation completion?
3 minutes
This timing is crucial for accurate results.
What is the time limit for reading strips for SLBL after incubation completion?
5 minutes
Reading after this time may lead to inaccurate results.
If the control line is not fully developed, what action should be taken?
Retest the sample
Ensures reliability of the test results.
Fill in the blank: An _______ C line indicates an invalid test.
absent or partial
Both conditions invalidate the test.
True or False: The test results can be read at any time after incubation.
False
There are specific time limits for reading the results.
What should be done if the C line is absent?
The sample must be re-tested
This is necessary for ensuring the accuracy of the test.
What is the temperature range for using Negative Control?
0-4.4° C
This temperature range is critical for maintaining the integrity of the control.
What should be done with previously negative tested raw milk if it’s not used within 72 hours?
Aliquot within 24 hours and freeze at -15° C or colder
This ensures the sample remains viable for testing.
How long can a thawed Negative Control be used after being frozen?
Use within 2 months
This is the maximum duration for which the control can remain viable after thawing.
What is the consequence of refreezing a Negative Control?
Do not refreeze
Refreezing can compromise the integrity of the control.
What action should be taken if the controls are out of range?
Do not proceed
This is essential for ensuring accurate test results.
What is the required action for thawed Negative Control for SLBL?
Requires a 3 minute centrifugation
Centrifugation is necessary to prepare the sample for testing.
What is the control value for Negative Control?
More negative than -600
This indicates the expected performance of the Negative Control sample.
What is a Positive Control in the context of the 2400 Form?
5 ppb Penicillin tablet rehydrated with 5 ml of negative control. Positive control is more positive than +400.
What is the storage recommendation for Positive Control?
Use within 48 hours when maintained at 0-4.4° C.
What should be done with Positive Control if not used within 48 hours?
Aliquot within 24 hours and freeze at -15° C or colder in a non frost-free freezer or in a Styrofoam container in a frost-free freezer, use within 2 months.
Can Positive Control be refrozen?
No, do not refreeze.
What should be done if Controls are out of range?
Do not proceed.
What is required for thawed Negative Control for SLBL?
Requires a 3 minute centrifugation.
What is the primary purpose of the Charm® 3 SL3 Beta-lactam Test?
To test for the presence of beta-lactam antibiotics in milk samples.
This summary test procedure is not for use in regulatory testing.
What should be referred to for the complete procedure of the Charm® 3 SL3 Beta-lactam Test?
The current Operator’s Manual.
What is the acceptable temperature range for the incubator during the test?
56 ± 1 °C.
What is the first step after labeling the test strip?
Shake the milk sample (or controls).
What must be done to the test strip before placing it in the incubator?
Peel the tape back to the ‘Peel to here’ line.
How much sample should be pipetted into the sample compartment?
300 uL.
What is the maximum incubation time for the test?
3.5 minutes.
What should be visually inspected after removing the tests from the incubator?
The C (control) line for even development.
In what mode should the test strip be inserted into the ROSA Reader or Charm EZ system?
Read-only mode.
What should be done if the test samples are invalid?
Re-test the invalid samples.
True or False: Visual interpretation is accepted in NCIMS Milk Programs.
False.
Fill in the blank: After incubating, the completed strip should be placed in the _______.
ROSA Reader or Charm EZ system.
