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Lab Test > Pipet Calibration Semi Annual > Flashcards

Pipet Calibration Semi Annual Flashcards

1
Q

What is the pipet volume for the semi-annual calibration?

A

300UL

This refers to the volume capacity of the pipette being calibrated.

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2
Q

What is the serial number of the pipet?

A

A68031022

This unique identifier helps track the specific pipette for calibration records.

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3
Q

What is the accuracy of the balance used in the calibration?

A

+0.001g

This indicates the precision of the balance in measuring weight.

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4
Q

What is the method used for volume testing?

A

Gravimetric Volume Testing

This method involves weighing a liquid to determine its volume.

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5
Q

What is the target volume for the gravimetric volume testing?

A

0.300 mL

This is the volume of deionized or distilled water used for testing.

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6
Q

What are the individual weight limits for the pipet calibration?

A

+/-5%: 0.285 - 0.315 g

This range indicates acceptable weight variations during testing.

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7
Q

Where is the pipet located?

A

Receiving Bay, Room # 1

This specifies the physical location of the pipette in the lab.

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8
Q

What was the temperature at the time of calibration?

A

25°C

Temperature can affect liquid density and measurement accuracy.

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9
Q

What is the average weight recorded during the calibration?

A

0.300 g

This average weight indicates that the pipette dispenses the correct volume.

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10
Q

What is the pass/fail result of the calibration?

A

Pass: V

This indicates that the pipette calibration met the required standards.

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11
Q

What is the next due date for calibration?

A

07/02/10

This indicates when the next calibration is scheduled.

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12
Q

Fill in the blank: The balance serial number is _______.

A

1117132085

This unique identifier is essential for maintaining quality assurance.

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13
Q

What is a Traceable Thermometer checked at annually?

A

Ice Point

The thermometer is tagged with the date, identification, temperature checked, and correction information.

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14
Q

What is the accuracy of the Digital Thermometer?

A

+/- 2°F / +/- 1°C

This refers to the precision in temperature measurement.

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15
Q

What is the resolution of the Digital Thermometer?

A

0.1°F / C

This indicates the smallest change in temperature that can be displayed by the thermometer.

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16
Q

What is the temperature range of the 6” Alcohol Thermometer?

A

58° to 300°F / -50° to 150°C

This defines the minimum and maximum temperatures that can be measured.

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17
Q

What is the sampling time for the Digital Thermometer?

A

1.5 seconds

This is the time it takes for the thermometer to provide a reading.

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18
Q

How often should the temperature of the incubator be checked?

A

Daily

Regular checks ensure that the incubator maintains the proper temperature for optimal conditions.

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19
Q

Where can the temperature be checked on the Charm EZ?

A

Above the Charm EZ label

This is the designated area for monitoring temperature on the device.

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20
Q

Where can the temperature be checked on an external incubator?

A

Back of the incubator beneath the hinges

This location is specified for external incubator monitoring.

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21
Q

If the temperature is out of range, whom should you contact?

A

Charm Technical Support

This ensures that any issues are addressed promptly by qualified personnel.

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22
Q

Fill in the blank: The Digital Thermometer uses a _______ battery.

A

1.5 volt button

This specifies the type of power source used for the thermometer.

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23
Q

What is the maximum allowable temperature for raw milk TC samples upon receipt?

A

4.4° C

Samples exceeding this temperature should not be tested.

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24
Q

What temperature must the sample be maintained below?

A

7° C

It is crucial to ensure the sample temperature remains below this limit.

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25
Q

What should be done if a raw milk TC sample is overfilled?

A

Do not accept the sample

Overfilled samples can lead to inaccurate testing results.

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26
Q

What is required for proper mixing of the sample?

A

Sufficient head space

Allowing head space is important to facilitate effective mixing.

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27
Q

What is the first instruction regarding the test strip container?

A

Always reseal container with this lid immediately after opening

This ensures the integrity of the reagents.

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28
Q

What is the storage requirement for the sealed container?

A

Refrigerate

Proper refrigeration is necessary to maintain reagent stability.

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29
Q

What should be recorded in the reader and on the daily antibiotic log sheets?

A

Lot # of the test strips

This tracking is essential for quality control and traceability.

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30
Q

What color should the desiccant be?

A

Blue

Indicates that the desiccant is functioning properly.

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31
Q

How should the test strips be prepared for use?

A

Set out number of strips to be used and place them in a dry labeled container at room temperature

This preparation helps to avoid moisture contamination.

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32
Q

What should be done with any leftover test strips at the end of the day?

A

Discard any leftover

This prevents the use of expired or contaminated strips.

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33
Q

What is the sample preparation time for the Beta-lactam Test?

A

Milk sample and controls 25 times in 7 seconds

This refers to the rapid preparation required for accurate testing.

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34
Q

What should be done to avoid foam when preparing the sample?

A

Allow foam/bubbles to settle or tilt sample to avoid foam

Proper handling is crucial for accurate results.

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35
Q

How soon should the sample be used after shaking?

A

Use within 3 minutes of shaking

Timeliness is important to maintain sample integrity.

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36
Q

What is the time limit for pipetting the sample after placing the first strip in the incubator for SL3?

A

Pipet sample within 1 minute 15 seconds

This is critical for ensuring accurate test results.

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37
Q

What is the time limit for pipetting the sample after placing the first strip in the incubator for SLBL?

A

Pipet sample within 2 minutes

Adhering to this timeframe is essential for accuracy.

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38
Q

What should each test strip be labeled with?

A

Sample identification such as sample #, manifest or route #, and indicate F (Front) or R (Rear) if applicable.

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39
Q

Where should the test strip be placed after labeling?

A

In the Incubator.

40
Q

What should you do before using the sample compartment of the test strip?

A

Peel the backing open to expose the sample compartment.

41
Q

What does ‘CHARM’ refer to in the context of the document?

A

CHARM SCIENCES INC.

42
Q

What is the proper angle to hold a pipet sample?

A

At an angle to avoid foam and bubbles

Holding the pipet at an angle helps to minimize the introduction of air and prevents foaming.

43
Q

How should a pipet be positioned when in use?

A

Keep pipet vertical

A vertical position ensures accurate measurement and avoids contamination.

44
Q

What is the maximum depth a pipet tip should be inserted into a sample?

A

No more than 1 cm

This prevents damage to the pipet and ensures consistent sample handling.

45
Q

What is the maximum time allowed to complete all samples in the Charm 3 SL3 Beta-lactam Test?

A

1 minute 15 seconds

46
Q

What is the incubation time for the Charm 3 SL3 Beta-lactam Test?

A

3 minutes

47
Q

What is the maximum incubation time for the test strips in the Charm 3 SL3 Beta-lactam Test?

A

3 minutes and 30 seconds

48
Q

How should the pipet be positioned when adding a sample to the strip?

A

Upright and vertically above the strip with the tip in either side well of the sample pad compartment

49
Q

What should you avoid touching when adding the sample?

A

The sponge

50
Q

What is the procedure after adding all samples to the strip?

A

Re-seal strip before starting 2nd test

51
Q

What should be done after pipetting all samples?

A

Close latch

52
Q

What starts automatically after closing the latch in the Charm 3 SL3 Beta-lactam Test?

A

Automatic timer

53
Q

Is the automatic timer required for the Charm 3 SL3 Beta-lactam Test?

A

No

54
Q

Fill in the blank: Use the _______ line as a guide.

A

Etched

55
Q

What is the maximum incubation time for the Charm SLBL Beta-lactam Test?

A

9 minutes

The incubation should ideally be for 8 minutes.

56
Q

What should be done with samples after placing the first test strip in the incubator?

A

Complete all samples within 2 minutes

This is crucial for maintaining test accuracy.

57
Q

What is the recommended position for the pipette during the test?

A

Upright and vertically above strip

The tip should be in either side well of the sample pad compartment.

58
Q

What should be avoided when adding the sample to the test strip?

A

Touching the sponge

This helps prevent contamination.

59
Q

What should be done before starting the second test?

A

Re-seal the strip

Ensures that the test conditions remain controlled.

60
Q

What action should be taken after pipetting all samples?

A

Close the latch

This is important to secure the test environment.

61
Q

What happens when the latch is closed after pipetting?

A

An automatic timer will start

The timer helps to track the incubation time.

62
Q

Is the automatic timer required for the Charm SLBL Beta-lactam Test?

A

No, it is not required

Manual timing can also be used.

63
Q

What is the purpose of the etched line in the test procedure?

A

Use as a guide

It helps in proper positioning of the pipette.

64
Q

What should be visually inspected in the beta-lactam tests?

A

C (control) line for full development

The control line indicates whether the test was valid or not.

65
Q

What does an absent C line or partial C line indicate?

A

An invalid test

In such cases, the sample must be re-tested.

66
Q

What is the time limit for reading strips for SL3 after incubation completion?

A

3 minutes

This timing is crucial for accurate results.

67
Q

What is the time limit for reading strips for SLBL after incubation completion?

A

5 minutes

Reading after this time may lead to inaccurate results.

68
Q

If the control line is not fully developed, what action should be taken?

A

Retest the sample

Ensures reliability of the test results.

69
Q

Fill in the blank: An _______ C line indicates an invalid test.

A

absent or partial

Both conditions invalidate the test.

70
Q

True or False: The test results can be read at any time after incubation.

A

False

There are specific time limits for reading the results.

71
Q

What should be done if the C line is absent?

A

The sample must be re-tested

This is necessary for ensuring the accuracy of the test.

72
Q

What is the temperature range for using Negative Control?

A

0-4.4° C

This temperature range is critical for maintaining the integrity of the control.

73
Q

What should be done with previously negative tested raw milk if it’s not used within 72 hours?

A

Aliquot within 24 hours and freeze at -15° C or colder

This ensures the sample remains viable for testing.

74
Q

How long can a thawed Negative Control be used after being frozen?

A

Use within 2 months

This is the maximum duration for which the control can remain viable after thawing.

75
Q

What is the consequence of refreezing a Negative Control?

A

Do not refreeze

Refreezing can compromise the integrity of the control.

76
Q

What action should be taken if the controls are out of range?

A

Do not proceed

This is essential for ensuring accurate test results.

77
Q

What is the required action for thawed Negative Control for SLBL?

A

Requires a 3 minute centrifugation

Centrifugation is necessary to prepare the sample for testing.

78
Q

What is the control value for Negative Control?

A

More negative than -600

This indicates the expected performance of the Negative Control sample.

79
Q

What is a Positive Control in the context of the 2400 Form?

A

5 ppb Penicillin tablet rehydrated with 5 ml of negative control. Positive control is more positive than +400.

80
Q

What is the storage recommendation for Positive Control?

A

Use within 48 hours when maintained at 0-4.4° C.

81
Q

What should be done with Positive Control if not used within 48 hours?

A

Aliquot within 24 hours and freeze at -15° C or colder in a non frost-free freezer or in a Styrofoam container in a frost-free freezer, use within 2 months.

82
Q

Can Positive Control be refrozen?

A

No, do not refreeze.

83
Q

What should be done if Controls are out of range?

A

Do not proceed.

84
Q

What is required for thawed Negative Control for SLBL?

A

Requires a 3 minute centrifugation.

85
Q

What is the primary purpose of the Charm® 3 SL3 Beta-lactam Test?

A

To test for the presence of beta-lactam antibiotics in milk samples.

This summary test procedure is not for use in regulatory testing.

86
Q

What should be referred to for the complete procedure of the Charm® 3 SL3 Beta-lactam Test?

A

The current Operator’s Manual.

87
Q

What is the acceptable temperature range for the incubator during the test?

A

56 ± 1 °C.

88
Q

What is the first step after labeling the test strip?

A

Shake the milk sample (or controls).

89
Q

What must be done to the test strip before placing it in the incubator?

A

Peel the tape back to the ‘Peel to here’ line.

90
Q

How much sample should be pipetted into the sample compartment?

A

300 uL.

91
Q

What is the maximum incubation time for the test?

A

3.5 minutes.

92
Q

What should be visually inspected after removing the tests from the incubator?

A

The C (control) line for even development.

93
Q

In what mode should the test strip be inserted into the ROSA Reader or Charm EZ system?

A

Read-only mode.

94
Q

What should be done if the test samples are invalid?

A

Re-test the invalid samples.

95
Q

True or False: Visual interpretation is accepted in NCIMS Milk Programs.

A

False.

96
Q

Fill in the blank: After incubating, the completed strip should be placed in the _______.

A

ROSA Reader or Charm EZ system.

Lab Test (13 decks)

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