Direct Microscopic Somatic Cell Count Flashcards
What is the maximum time unpreserved samples can be tested after initial collection?
72 hours
What are the preservatives that allow samples to be tested up to 7 days after collection?
0.02% 2-bromo-2-nitropropane-1,3-dio (Bronopol™) or 0.05% potassium dichromate (K2Cr2O7)
What is the required face velocity for a functional fume hood?
100 ft/min
What are the dimensions of the clean microscope slides used in the procedure?
2.54 x 7.62 cm
What is the diameter of the areas delineated on the microscope slides?
11.28 mm
What is the purpose of the metal syringe in the process?
For rapid and convenient transfer of 0.01 mL of milk
What is the acceptable weight delivery range for the metal syringe?
0.0103 ± 0.0005 g
What must be done annually for the fume hood?
Check, maintain records, and tag unit
What identification is required for the metal syringe?
Etched identification or imprinted serial number
Fill in the blank: Samples may be run up to _____ after initial collection if preserved.
7 days
True or False: Micropipettors are used for transferring more than 0.01 mL of milk.
False
What is the average weight to be checked for accuracy with the metal syringe?
0.0103 g
What is the significance of the accuracy check date for the metal syringe?
To ensure proper functioning and reliability
What is the average weight specified for accuracy check in item 6.e.4?
0.0103 +0.0005g
This is based on the average of 10 consecutive weighings with milk.
What should be done if using Artel PCS according to CP item 6.e.5?
See CP item 6.e.5
Specific instructions for using Artel PCS are outlined in that section.
What is required for the micropipettor used in the procedure?
Etched with identification (imprinted serial number acceptable); tag with accuracy check date
This ensures traceability and compliance with accuracy checks.
What type of needle is suitable for spreading milk film?
Dissecting Needle, Bent Point
This needle design facilitates even spreading of the film.
What temperature range should the drying device maintain?
40-45°C
This temperature range is crucial for effective drying.
What is required to monitor the temperature of the drying device?
Temperature measuring device
Accurate temperature monitoring ensures proper drying conditions.
What tools are required for dipping and holding slides?
Forceps or Slide Holder
These tools help in handling slides without contamination.
What should staining jars or trays have?
Tight fitting covers
This helps to prevent evaporation and contamination of solvents and stains.
What is the recommended storage condition for slides?
Clean, dust-free insect-proof boxes, cases or files
Proper storage conditions are necessary to maintain slide integrity.
What type of microscope is specified for use?
Binocular with 1.8 mm oil immersion objective, rack and pinion sub-stage, condenser with iris diaphragm
This microscope configuration is essential for detailed observation.
What ocular magnification is recommended for the microscope?
10X (12X or 12.5X), Huygenian or wide-field
These magnifications provide a suitable field of view for analysis.