General Dairy Bacteriology Flashcards

1
Q

What are bacteria?

A

Single-celled organisms that can only be seen with a microscope

All processes needed for life occur within the cell.

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2
Q

What is the magnification required to see bacteria?

A

1,000X

A microscope is necessary to observe bacteria.

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3
Q

In what unit are bacteria measured?

A

Microns

1 micron = 1/1,000 mm = 1/25,000 inch.

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4
Q

What are the three categories of bacteria?

A
  • Beneficial
  • Pathogenic
  • Spoilage

Each category has distinct roles and impacts, especially in the dairy industry.

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5
Q

What are beneficial bacteria responsible for?

A

Food fermentations (e.g. cheese)

These bacteria play a crucial role in dairy processing.

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6
Q

What do pathogenic bacteria cause?

A

Disease

These bacteria pose health risks in food products.

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7
Q

What do spoilage bacteria cause?

A

Food products to break down

This is a primary concern in the dairy industry.

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8
Q

What shape are cocci bacteria?

A

Spherical

Cocci can occur as single cells, pairs, chains, or clusters.

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9
Q

What is the size range for cocci bacteria?

A

0.4-1.5 microns

This size range is typical for cocci found in milk.

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10
Q

What shape are bacilli bacteria?

A

Rod shaped

Bacilli can occur as single cells, pairs, or chains.

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11
Q

What is the size range for bacilli bacteria?

A

0.5-30 microns

This range indicates the variability in bacilli size.

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12
Q

How are individual bacteria named?

A

By Genus and species (e.g. Bacillus cereus)

This naming convention applies to all living organisms.

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13
Q

What are the unusual shapes of bacteria that are less common in milk?

A

Spiral, helical, or club shaped

These shapes are generally not very prevalent in dairy products.

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14
Q

What is a common method to visualize bacteria in milk?

A

Staining a dried milk smear with a specific ‘milk-stain’

An example of a milk-stain is the Levowitz-Weber stain.

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15
Q

What is the primary classification of most bacteria?

A

Gram-positive or Gram-negative

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16
Q

What is the purpose of the Gram stain procedure?

A

To view and differentiate bacteria under a microscope

17
Q

What are endospores?

A

Protective, dormant structures that allow an organism to survive under adverse conditions

18
Q

What triggers the formation of bacterial spores?

A

Unfavorable conditions such as lack of nutrients

19
Q

What color do Gram-positive bacteria stain?

A

Blue

20
Q

What color do Gram-negative bacteria stain?

A

Red

21
Q

What are some examples of Gram-positive bacteria?

A
  • Bacillus
  • Streptococcus
  • Staphylococcus
22
Q

What are some examples of Gram-negative bacteria?

A
  • Pseudomonas
  • E. coli & other coliform bacteria
23
Q

What does Gram-variable mean?

A

Stains blue or red depending on conditions; most are Gram-positives

24
Q

What are some conditions that bacterial spores resist?

A
  • Heat
  • Drying
  • Nutrient deprivation
  • Presence of chemicals or sanitizers
25
Q

Which type of bacteria do not survive pasteurization?

A

Gram-negative bacteria

26
Q

What type of spores are produced by only a few select groups of bacteria?

A

Endospores

27
Q

What type of bacteria are commonly spore-forming and important to dairy?

A

Gram-positive rods

28
Q

Where are spores commonly found?

A
  • Soil
  • Manure
  • Other environmental sources
29
Q

Fill in the blank: Spores can remain dormant for ______ periods of time.

A

extended

30
Q

True or False: Spores are produced by many different groups of bacteria.

A

False

31
Q

What happens when conditions become favorable for spores?

A

A spore can germinate and return to an actively growing state

32
Q

What is the significance of spore-forming bacteria in dairy spoilage?

A

Gram-negative bacteria spoil milk faster under refrigeration compared to Gram-positive spoilage organisms