Bactosomatic

Question 1

What is the first step to prepare the Bactosomatic unit for samples?

Answer 1

Go to measure mode and perform a self-check, which takes about 10 minutes.

Question 2

What are the limits for running blanks (distilled water) under the Blank SCC Product Channel?

Answer 2

Mean must be ≤3 and all individual measures must be <5.

Question 3

What is the acceptable result for blanks under the Blank IBC Product Channel?

Answer 3

All results must be ≤ 2 CFU.

Question 4

What is the procedure to run a BCS Control Sample?

Answer 4

Use 30mL distilled water in a universal vial, transfer 7.5 mL to BCS Control vial, agitate, dump back into universal vial, rinse BCS vial, and run under BCS product channel.

Question 5

What is the deviation limit for the BCS Control Sample?

Answer 5

Deviation should be <10%.

Question 6

What is the procedure for running the FMA (Somatic Cell) Control sample?

Answer 6

Shake the FMA sample well and run it under the FMA product channel, entering the number off the FMA Control box.

Question 7

What is the deviation limit for the FMA Control Sample?

Answer 7

Deviation should be <10%.

Question 8

What should be done every 3-4 hours during operation?

Answer 8

Run the Extra Clean program with at least 60mL of bactoscan rinse solution heated to 40C.

Question 9

What is the final step at the end of the day?

Answer 9

Select Stop mode and run a small universal vial of end of day solution.

Question 10

What must be done with the day’s results after testing?

Answer 10

Export the day’s results onto a thumb drive.

Question 11

What is the threshold for recording SCC results?

Answer 11

Record SCC as <100,000 if results are less than 100,000.

Question 12

What is the acceptable CV limit for SCC in the Repeatability IBC-SCC program?

Answer 12

CV of <3% at 500,000 cells.

Question 13

What is the limit for IBC in the Repeatability IBC-SCC program?

Answer 13

SR of ≤0.04 at an IBC count of >200.

Question 14

What is the procedure for the Monthly Rinse/Sheath Chamber Clean program?

Answer 14

Run while in stop mode using 500ml of fresh end of day solution.

Question 15

What is the procedure for running a PCS Sample?

Answer 15

Put PCS Bottle on magnetic stirrer for 10 minutes, mix 5 drops of PCS solution with 20 mL of water in a vial, and run under PCS IBC Program.

Question 16

What are the acceptable results for the Carry over IBC-SCC program?

Answer 16

Carry over results should be <1%.

Question 17

What maintenance is required for the inline filter?

Answer 17

Remove filter, run under tap water, rinse with distilled water, and replace.

Question 18

What is the formula for calculating the Microscopic Factor?

Answer 18

Microscopic Factor = 3.1416 * Diameter

The diameter should be in the same unit as the final result you want.

Question 19

What is the value of the Microscopic Factor when the diameter is 0.190?

Answer 19

Microscopic Factor = 3.1416 * 0.190 = 0.5978

This result is derived from the formula provided.

Question 20

What is the calculated value of the Microscopic Factor when the diameter is 0.1134?

Answer 20

Microscopic Factor = 3.1416 * 0.1134 = 0.3562

This calculation illustrates how the diameter affects the Microscopic Factor.

Question 21

What is the final value of the Microscopic Factor if the diameter leads to a calculation of 350,000?

Answer 21

Microscopic Factor = 350,000

This represents a specific case where the diameter is not explicitly stated.

Question 22

What is the formula for calculating the Single Strip Factor (SSF)?

Answer 22

SSF = 11.28 * Diameter

Similar to the Microscopic Factor, this formula uses diameter as a key input.

Question 23

What is the value of the Single Strip Factor when the diameter is 0.190?

Answer 23

SSF = 11.28 * 0.190 = 2.1432

This specific calculation shows the relationship between diameter and SSF.

Question 24

What is the Working Factor based on the Microscopic Factor value of 35,000?

Answer 24

Working Factor = 35,000

The Working Factor is derived from the Microscopic Factor calculations.