Bactosomatic Flashcards
What is the first step to prepare the Bactosomatic unit for samples?
Go to measure mode and perform a self-check, which takes about 10 minutes.
What are the limits for running blanks (distilled water) under the Blank SCC Product Channel?
Mean must be ≤3 and all individual measures must be <5.
What is the acceptable result for blanks under the Blank IBC Product Channel?
All results must be ≤ 2 CFU.
What is the procedure to run a BCS Control Sample?
Use 30mL distilled water in a universal vial, transfer 7.5 mL to BCS Control vial, agitate, dump back into universal vial, rinse BCS vial, and run under BCS product channel.
What is the deviation limit for the BCS Control Sample?
Deviation should be <10%.
What is the procedure for running the FMA (Somatic Cell) Control sample?
Shake the FMA sample well and run it under the FMA product channel, entering the number off the FMA Control box.
What is the deviation limit for the FMA Control Sample?
Deviation should be <10%.
What should be done every 3-4 hours during operation?
Run the Extra Clean program with at least 60mL of bactoscan rinse solution heated to 40C.
What is the final step at the end of the day?
Select Stop mode and run a small universal vial of end of day solution.
What must be done with the day’s results after testing?
Export the day’s results onto a thumb drive.
What is the threshold for recording SCC results?
Record SCC as <100,000 if results are less than 100,000.
What is the acceptable CV limit for SCC in the Repeatability IBC-SCC program?
CV of <3% at 500,000 cells.
What is the limit for IBC in the Repeatability IBC-SCC program?
SR of ≤0.04 at an IBC count of >200.
What is the procedure for the Monthly Rinse/Sheath Chamber Clean program?
Run while in stop mode using 500ml of fresh end of day solution.
What is the procedure for running a PCS Sample?
Put PCS Bottle on magnetic stirrer for 10 minutes, mix 5 drops of PCS solution with 20 mL of water in a vial, and run under PCS IBC Program.
What are the acceptable results for the Carry over IBC-SCC program?
Carry over results should be <1%.
What maintenance is required for the inline filter?
Remove filter, run under tap water, rinse with distilled water, and replace.
What is the formula for calculating the Microscopic Factor?
Microscopic Factor = 3.1416 * Diameter
The diameter should be in the same unit as the final result you want.
What is the value of the Microscopic Factor when the diameter is 0.190?
Microscopic Factor = 3.1416 * 0.190 = 0.5978
This result is derived from the formula provided.
What is the calculated value of the Microscopic Factor when the diameter is 0.1134?
Microscopic Factor = 3.1416 * 0.1134 = 0.3562
This calculation illustrates how the diameter affects the Microscopic Factor.
What is the final value of the Microscopic Factor if the diameter leads to a calculation of 350,000?
Microscopic Factor = 350,000
This represents a specific case where the diameter is not explicitly stated.
What is the formula for calculating the Single Strip Factor (SSF)?
SSF = 11.28 * Diameter
Similar to the Microscopic Factor, this formula uses diameter as a key input.
What is the value of the Single Strip Factor when the diameter is 0.190?
SSF = 11.28 * 0.190 = 2.1432
This specific calculation shows the relationship between diameter and SSF.
What is the Working Factor based on the Microscopic Factor value of 35,000?
Working Factor = 35,000
The Working Factor is derived from the Microscopic Factor calculations.
