Photosynthesis Experiments - Chromatography (Topic 5A)

Question 1

Mobile phase in chromatography

Answer 1

where molecules can move

Question 2

Stationary phase in chromatography

Answer 2

where molecules can’t move

Question 3

How are pigments identified

Answer 3

by comparing Rf values

Question 4

What should the leaves be grinded up with in TLC

Answer 4

anhydrous sodium sulfate

Question 5

What should be added to the grinded up mixture

Answer 5

propanone

Question 6

What should be added to the test tube with the grinded mixture in

Answer 6

petroleum ether

Question 7

What should happen once the petroleum ether has been added

Answer 7

the test tube should be shook gently

Question 8

Which layer should you transfer into another test tube with some sodium sulphate

Answer 8

top layer

Question 9

What should the line be drawn in

Answer 9

pencil to stop it interfering with the pigments

Question 10

what is the point of origin

Answer 10

where the initial spot of pigment is

Question 11

Rf value equation

Answer 11

distant travelled by spot/distance travelled by solvent