Photosynthesis Experiments - Activity of Dehydrogenase (Topic 5A)

Question 1

Which part of the leaves need to be removed

Answer 1

Any tough stalks

Question 2

What should the leaves be grinded up with

Answer 2

chilled isolation solution

Question 3

What should happen when you have your solution

Answer 3

filter it

Question 4

What should the liquid be transferred to

Answer 4

centrifuge tubes to be centrifuged at a high speed for 10 minutes and then get rid of the liquid from the top

Question 5

What should the pellets be resuspended in

Answer 5

fresh, chilled isolation solution, store it in ice for the rest of the experiment

Question 6

What equipment should be set up

Answer 6

a colourimeter

Question 7

How should the test tube rack be set up

Answer 7

a set distance from a bench lamp

Question 8

What should be added to a test tube

Answer 8

a set volume of chloroplast extract and a set volume of DCPIP and mix

Question 9

a set volume of chloroplast extract and a set volume of DCPIP mixture should be added to what

Answer 9

a clean cuvette so that the absorbance can be read