Investigating Mitosis (Topic 2A) Flashcards
What can be used to observe mitosis
root tips
What does the boiling tube need to contain
1M hydrochloric acid
What temperature does the water bath need to be
60 degrees Celsius
How long does the root tip need to be incubated for
5 minutes
How do you rinse the root tip
use a pipette
How much do you cut off root tip
2 mm
How do you break open the root tip
use a mounted needle
What needs to be added to highlight the sample and protect it
a stain and a coverslip
Where do you clip your microscope slide
onto the stage
which objective lens should you use at the start
the lowest powered one
How do you move the stage
use the coarse adjustment knob
How do you view the slide
use the eyepiece lens
How do you adjust the focus of the slide
use the fine adjustment knob
Mitotic index equation
number of cells with visible chromosomes/ total number of cells observed
