Enzyme Controlled Reactions (Topic 1A)

Question 1

Ways to measure rate of enzyme controlled reaction

Answer 1

Measure how fast a product is made or measure how fast a substrate is broken down

Question 2

What does the enzyme catalase do

Answer 2

catalyses the breakdown of hydrogen peroxide to water and oxygen

Question 3

Apparatus needed to measure how fast a product is made

Answer 3

boiling tube, delivery tube, bung, hydrogen peroxide solution and catalase enzyme, trough of water, upside down measuring cylinder.

Question 4

What do the boiling tubes need to contain

Answer 4

same volume and concentration of hydrogen peroxide, with an equal volume of buffer

Question 5

What does a PH buffer do

Answer 5

keeps the PH constant

Question 6

Where do the boiling tubes go

Answer 6

in a water bath at different temperatures

Question 7

what enzyme is added to boiling tubes

Answer 7

catalyse, then add bung and measure oxygen produced in first minute with a stopwatch

Question 8

How do you find an average volume produced

Answer 8

Repeat each temperature three times

Question 9

How do you calculate average rate of reaction

Answer 9

divide the volume of oxygen produced by time taken

Question 10

Enzyme used to investigate how fast a substrate is broken down

Answer 10

amylase

Question 11

What does the enzyme amylase do

Answer 11

it catalyses the breakdown of starch to maltose

Question 12

Apparatus used to investigate how fast a substrate is broken down

Answer 12

spotting tile, dropping pipette, drop of iodine in potassium iodide, test tube and starch solution and amylase solution

Question 13

How to test how fast a substrate is broken down

Answer 13

test how long it takes for colour change to occur from orange to blue black