Investigating Selection (Topic 4B)

Question 1

What is the bacteria used grown in

Answer 1

liquid broth

Question 2

What is used to transfer the bacteria from the broth to an agar plate

Answer 2

a sterile pipette

Question 3

How do you spread the bacteria over the plate

Answer 3

a sterile plastic spreader

Question 4

What are the paper disked soaked with

Answer 4

different antibiotics

Question 5

How do you place the discs on the plate

Answer 5

sterile forceps

Question 6

What should be added as part of the test

Answer 6

a negative control

Question 7

How is the lid kept on

Answer 7

it is taped on

Question 8

How are they incubated

Answer 8

inverted, kept at 25 degrees Celsius for 48 hours

Question 9

What is the clear patch called

Answer 9

inhibition zone, the larger the zone. the more bacteria were inhibited from growing

Question 10

What are aseptic techniques used for

Answer 10

to prevent contamination

Question 11

Aseptic techniques

Answer 11

disinfect surfaces, use sterile equipment, minimise time spent with lid off the agar, work near a Bunsen Burner and briefly flame the neck of the glass container of broth