Photosynthesis and etc Flashcards
What is the photosynthesis equation?
Carbon dioxide + water —-> glucose + oxygen
What is the photosynthesis symbol equation?
6CO2 + 6H2O —-> C6H12O6 + 6O2
What is the importance of photosynthesis?
Provides energy all living things require
Produces O2
Chlorophyll converts it to chemical energy
Plants use molecules to produce ATP for respiration
Non-photosynthetic organisms feed on molecules produced by plants
What are the features of a plant cell?
Amyloplast SER RER Nucleus Ribosomes Cell wall Golgi apparatus Chloroplast Vacuole Cell membrane Mitochondria
What makes leaves good at photosynthesis?
Large SA Thin Transparent cuticle Palisade cells long, thin + tightly packed Many stomata Air spaces
Why is it it good that leaves have a large SA?
Absorb max light
Why is it it good that leaves are thin?
Efficient gas exchange
Why is it it good that leaves have a transparent cuticle?
Allows light to upper mesophyll
Why is it it good that leaves have palisade cells that are long,thin + tightly packed?
Max light absorption
Why is it it good that leaves have many stomatas?
Efficient gas exchange + short diffusion distance
Why is it it good that leaves have air spaces?
Rapid gas exchange
What structures are present in a chloroplast?
Outer membrane Inner membrane Thykaloid sticks Stroma Grana Intergranal lamellae
What do thykaloid discs contain?
Chlorophyll
What is grana?
Stacks of thykaloid sticks
What is the stroma?
Fluid-filled matrix around grana
Where does the LIDR take place?
Stroma
Where can starch grains be found?
Stroma
What do some thykaloid sticks have?
Tube shaped extensions
What does the tube shaped extensions connect?
Grana
What is it called when the tube shaped extensions join the grana?
Intergranal lamellae
What is the function of granal membranes?
Large SA for chlorophyll, electron carriers + enzymes
What is the function of the network of proteins in grana holding chlorophyll in specific way?
Allows for max light absorption
What is the function of the fluid in the stroma containing enzymes?
Needed for LDR
Allows to maintain optimum enzyme conc
What is the function of chloroplasts containing DNA + ribosomes?
Can quickly + easily manufacture proteins needed for photosynthesis
What is the function of granal membranes having ATP synthase channels?
Catalyse production of ATP
Selectively permeable
What is the function of stroma surrounding grana?
Products from LDR easily diffuse into stroma for LIDR
What is the law of limiting factors?
When a process depends on two or more factors, the rate of that process is the limited by the factor which is in shortest supply
What factors affect photosynthesis?
Light intensity Availability of H2O Temperature Availability of chlorophyll Availability of CO2
What is the compensation point?
When the rate of photosynthesis = rate of respiration
What does the graph look like for CO2 conc against rate of photosynthesis?
Increases then plateaus
What does the graph look like for chlorophyll against rate of photosynthesis?
Increases then plateaus
What does the graph look like for temperature against rate of photosynthesis?
Increases the decreases
Upside down “U”
Why is the temperature graph different?
Increases till optimum
More KE so more successful collisions
Too hot so breaks H bonds so active site denatures
What are the three main stages in photosynthesis?
Capturing of light energy
LDR
LIDR
What does chlorophyll A absorb?
Mainly red-orange + blue-violet part of visible light spectrum
Describe the first step of LDR
Making ATP
Photon of light absorbed = boosts energy level of pair of chlorophyll electrons
Excited electrons leave chlorophyll molecule
Chlorophyll molecule oxidised
ETC gains electrons = photoionsiation
Describe 2nd step of LDR
ETC and Photosystems
Excited electrons taken up ETC Move along ETC from PS2 Series of redox reactions Each carrier slightly lower energy than previous So electrons lose energy at each stage
Why do the electrons move?
Each carrier has slightly higher affinity for electrons than last
This draws electrons along chain to PS1
What are photosystems ?
Functional and structural proteins in photosynthesis
What do photosystems do?
Absorb light + transfer energy and electrons
Where are photosystems found?
Thykaloid membrane
What happens to the energy that is lost?
Used to synthesise ATP in photophosphorylation
Why do excited electrons pass through 2 photosystems?
Further absorbed light energy = increases energy of electrons
Makes energy sufficient for reduction of NADP to NADPH
Describe the 2nd step 2.0 of LDR
NADP+
NADP final acceptor in ETC so becomes reduced
Reduced to NADPH
NADPH stores electrons + protons till they are transferred to LIDR
What is NADP+?
Coenzyme that accepts protons + electrons
Describe the 3rd step of LDR
Photolysis of water
H2O split from light energy absorbed by chlorophyll A
When absorbs light chlorophyll oxidised
Electrons produced from photolysis of water used to reduce chlorophyll
What is the equation for photolysis of water?
2H2O —-> O2 + 4H+ + 4e-
Why are protons very important in LDR?
Taken up by NADP = final acceptor
Vital for LDR
Why does LDR have 2 photosystems?
So electrons have enough energy to be picked up by NADP+ (coenzyme)
What is chemiosmosis?
The process of electrons flowing down the electron transport chain creating a proton gradient across the membrane to drive ATP synthesis
Where does the LDR
Thykaloid sticks
What is the LIDR called?
Calvin Cycle
What products of LDR are needed for LIDR?
NADPH - used to form simple sugar
ATP - hydrolysis of ATP provides energy
What are the three stages of LIDR?
Carbon fixation
Reduction
Regeneration
Brief description of carbon fixation stage (1)
CO2 combined with RuBP to make 2x GP
What is RuBP?
Ribulose bisphosphate
What is GP?
Glycerate 3-phosphate
Describe carbon fixation stage (1) in LIDR
CO2 diffuses into stroma through stomata Reacts with 5C RuBP Catalysed by rubisco Forms unstable 6C compound Immediately broken down into 2x GP
What is rubisco?
Ribulose bisphosphate carboxylase
Brief description of reduction stage (2)
ATP and NADPH used to reduce GP to TP
What is TP?
Triose phosphate
Describe reduction stage (2) in LIDR
NADPH (from LDR) reduces 3C GP into 2x 3C TP using ATP (from LDR)
NADP reformed + returned to LDR
Brief description of regeneration stage (3)
RuBP regenerated
Describe of regeneration stage (3) in LIDR
TP has 2 uses:
5/6 - regenerates RuBP using ATP (from LDR)
1/6 - forms organic substance
How many times does the Calvin Cycle need to turn to produce one glucose molecule?
6
How many ATPs and NADPHs does the Calvin Cycle need to make one glucose molecule?
18 ATP
12 NADPH
Describe what happens in the chemiosmotic theory?
Proton pumps produces H+ from stroma into thykaloid membrane
Active transport
H+ pass through chemiosmotic channel to stroma
Diffusion
Produces ATP
Catalyses combo of ADP + Pi as they pass through
Where is the energy needed for active transport taken from in the chemiosmotic theory?
Energy from excited electrons that pass along ETC
Produces ATP
What happens as H+ pass through chemiosmotic channel?
Change the structure of the enzyme
What type of protein in the proton pump in the chemiosmotic theory?
Intrinsic protein