Photosynthesis and Cellular Respiration (chapters 5-6) Flashcards
ATP
Adeno-Triphosphate
ADP
Adeno-Diphosphate
Sources of Phosphate
- Food
- Sunlight
Reverse of Photosynthesis
Cellular Respiration
Purpose of Photosynthesis
Convert solar energy into chemical energy, stored for future use
Location of Photosynthesis
Takes place in Eukaryotes in the membrane bound organelle: The Chloroplast
Where does chloroplast get it’s green pigment from?
Chlorophyll
How does Photosynthesis start?
Chlorophyll absorbs wavelengths of light required to begin photosynthesis
Where is Chlorophyll found?
Thylakoid Membrane within the Chloroplast
Stoma
A tiny pore used for gas exchange in plants
Endosymbiosis
Theory that eukaryotic cells came from prokaryotic cells
Chloroplast Contents:
- Thylakoid
- Stroma
- DNA and Ribosomes
Thylakoid
Flattened structure, stacked into structure called GRANA
Stroma
Liquid interior of organelle
C3 Photosynthesis Phases
- Light dependant phase
- Light independant phase (Calvin Cycle)
Light Dependant Phase Location
Occurs in thylakoid membrane of Chloroplast, mostly in Mesophyll Cells
Light Independant Phase Location
Occurs in the Stroma of the Chloroplast, Mosly in Bundle Sheat Cells
Light Dependant Phase
- Chlorophyll traps light energy
- Water is splt to produce O2
- NADP+ picks up H+ ions and electrons to become NADPH
- ATP synthase, an enzyme complex found in the thylakoid membrane, converts ADP and Phosphate to ATP
Light Independant Phase
- Carbon fixation – which refers to the conversion of CO2 and RuBP into 3-PGA.
Carbon from the inorganic CO2 is ‘fixed’ into an organic compound.
Rubisco is responsible for taking carbon from an inorganic, gaseous form (CO2) and
incorporating it into an organic compound (3-PGA) - Reduction – NADPH donates electrons to an intermediate three-carbon molecule in the cycle to produce G3P
- Regeneration – the RuBP molecules needed to start the cycle again are reproduced.
Does Light Independant Phase require light
Light independant phase does not require light, but does require the outputs of the light dependant phase, Thereform the Light Independant phase ceases shortly after the Light Dependant phase
RuBisCo
Enzyme in the Calvin Cycle, incorporates CO2 into plants during Photosynthesis
Stages of Calvin Cycle
1) RuBisCo incorporates Carbon Dioxide into an organic molecule (3-PGA)
2) Organic molecule is reduced using electrons supplied by NADPH
3) RuBP, the molecule that starts the cycle, regenerates so that the cycle can start again
Problem with RuBisCo
If stomata close to prevent water loss, O2 builds up within the Stomata, causing:
RuBisCo to add O2 instead of CO2 to RubP
Carbon Fixation
CO2 is ‘fixed’ from it’s organic form to organic molecules
How many cycles must the Calvin cycle go through to produce 6 Carbon Molecule
6 Times
Stomata
Pores on a leaf surface, allow entry and exit of gases
PEP
Phosphoenolpyruvate (C3 Compound)
Factors Affecting Rate of Photosynthesis
1) Light Intensity
2) Light Colour
3) Water Availability
4) Temperature
5) CO2 Concentration
Light Intensity (Photosynthesis Factor)
As light becomes more intense, Rate of Photosynthesis also rises, (up to the point it levels off)
At low intensity, plants may be using more oxygen than it produces
Lower than rate of cellular respiration at low light
Light Compensation Point
Light intensity at which the rate of photosynthesis matyches that of cellular respiration (produces and consumes equal amounts of oxygen)
Light Colour (Photosynthesis Factor)
Plants mostly absorb SHORT blue and LONG red wavelengths in light spectrum
Barely absorbs green wavelengths (centre of wavelength)
Green light is the only colour not absorbed by Chlorophyll, therefore passingh through, being visible
Water Availability (Photosynthesis Factor)
Photosynthesis requires water molecules
Stomata can lose turgor (becoming flacid), preventing Gas exchange
Temperature (Photosynthesis Factor)
For most plants, optimal temperature is approx 35oC
Molecules move faster when hotter
If too high, or too low, rate of respiration decreases
CO2 Concentration (Photosynthesis Factor)
At higher concentrations, rate of Photosynthesis is greater
Concentration in atmosphere is 0.04%
Optimal concentration is 0.1% - no effect past 0.1%
Aerobic Cellular Respiration Stages
1) Glycolysis
2) Krebs Cycle
3) Electron Transport Chain
Krebs Cycle Location
Matrix (fluid) of mitochondrion
Glycolysis Location
Cytosol
Glycolysis Stages
- Investment Stage - uses two ATP
- Pay off stage - produces four ATP
Glycolysis
Literally sugar breaking
Does Glycolysis use O2
No
Does Krebs Cycle use O2
No, though can’t take place without presence of O2
Stages of Krebs
- Oxidization of Pyruvate - forms Acetyl COA
- Krebs Cycle (Krebs) - forms NADH and H+ (extra)
How much ATP does Aerobic Respiration Yield
30 - 32 (not 36-38)
Electron Transport Chain Location
Takes place on Cristae of the fluids of inner mitochondrial membrane
Krebs Cycle description
All carbon and oxygen from pyruvate is released as CO2
This creates more high energy coenzyme: 2ATP, 8NADH and 2FADH2
Electron Transport Chain description
As hydrogens pass through, they lose energy (H+ ions)
H+ ions diffuse back into matrix thorough ATP synthase, which rotates, creating ATP
Oxygen accepts H+ ions, forming water
Anaerobic Respiration
Allows to still get a little ATP without oxygen
What does Anaerobic Respiration Yield?
2 ATP
2 Pyruvate
2 NADH Molecules
Anaerobic Fermentation in Animals (Lactic Acid Fermentation)
Mammals undergo Anaerobic Respiration when their demand for energy outstrips their ability to obtain adequate oxygen to undergo Aerobic Respiration
Glucose broken down to produce Pyruvate, then converted to Lactic Acid
Net gain of 2 ATP
Anaerobic Fermentation in Yeast (Alcohol Fermentation)
Glucose first broken down to produce Pyruvate, then converted to Ethanol and Carbon Dioxide
Factors affecting Rate of Cellular Respiration
1) Temperature
2) Glucose Availability
3) Oxygen Availability (Aerobic Only)
4) ATP Demand
Temperature (Cellular Respiration Factor)
Cellular Respiration Catalyzed by Enzymes
Optimal Temperature for Cellular Respiration in huimans is 36.7oC
Enzymes Denature if too hot or too cold
Glucose Availability (Cellular Respiration Factor)
The higher the concentration of glucose, the greater the rate of cellular respiration is
This is because glucose is the preffered input for Cellular Respiration
At very high rates of glucose, respiration will reach it’s max rate, due to enzymes becoming saturated with substrate
Oxygen Availability (Aerobic Only) (Cellular Respiration Factor)
As oxygen is an input of Aerobic Respiration, a lack of oxygen will cause the rate of Cellular Respiration to decrease
ATP Demand (Cellular Respiration Factor)
ATP is the greatest factor affecting rate of respiration
Lack of ATP causes respiration to slow
Role of RuBisCo (Photosynthesis)
Rubisco is an enzyme within the light independent stage of photosynthesis. Rubisco uses 3 carbon dioxide molecules and 3 five-carbon molecules (RuBP) in order to produce 6 three carbon molecules (known as 3-PGA)
The 6 3-PGA molecules are then converted by ATP and NADPH into 6 three carbon molecules called G3P
One G3P molecule is used in the production of Glucose
and the remaning 5 are recycled (through ATP) to regenerate 3 x RuBP to recontinue the calvin cycle
Examples of C3 Plants
Soybeans
Oats
Wheat
Rice
ATP Synthase
an enzyme in the inner mitochondrial membrane that uses the concentration gradient of H+ to synthesise ATP from ADP and Phosphase
Photorespiration
When RuBisCO binds O2 instead of CO2
- Disrupts Photosynthesis (CO2 loses binding opportunity)
- Less photosynthesis -> less glucose -> less opportunity for plants to grow
CAM Plants
plants that minimise photorespiration by separating initial carbon fixation and the remainder of the Calvin cycle
C3 Plants
plants with no evolved adaptation to minimise photorespiration
C4 Plants
plants that minimise photorespiration by separating initial carbon fixation and the remainder of the Calvin cycle over space