Photosynthesis Flashcards
Photosynthesis is a _____ process.
Physico-chemical process
Photosynthesis is a physics-chemical process by which they use light energy to drive the synthesis of -
Organic compounds
Which simple experiment proves that photosynthesis occurs only in the green parts of leaves in the presence of light -
To look for starch formation in 2 leaves -variegated leaf or leaf that was partially covered with black paper
Which exp proves that CO2 was req for photosynthesis ?
A part of leaf is enclosed Ina test tube containing some KOH soaked cotton (which absorbs CO2 ) . On testing for the presence of starch , only the exposed part tested positive .
______in the year _____ revealed the essential role of air in the growth of green plants .
Joseph priestly
1770
_____ discovered O2 in the year __.
Joseph priestly
1774
Joseph priestly in the year 1770 revealed -
Essential role of air in the growth of green plants .
_____observed that a candle burning in a closed jar , soon gets extinguished .
Priestly
What was Joseph priestly’s hypothesis ?
Plants restore to air whatever breathing animals and burning candles remove .
Using a similar setup as the one used by PRIESTLY ,but by placing it once in the dark and once in the sunlight ,_____ showed that SUNLIGHT IS ESSENTIAL TO THE PLANT PROCESS (that purified the air )
Jan ingenhouz
______ in an elegant experiment with an ______ plant showed that in bright sunlight , small bubbles were formed around the green parts while in the dark they did not .
Ingenhouz
Who showed that it is ONLY THE GREEN PARTS OF THE PLANTS THAT COULD RELEASE OXYGEN .
JAN INGENHOUZ
What did jan engenhouz show-
2 things
Sunlight is essential to photosynthesis.
Only green plant parts release oxygen
In jan ingenhouzs’s experiment with an aquatic plant , small bubbles formed around the green parts were of -
O2
In year ____ , ______ provided evidence for production of glucose when plants grow .
1854
Julius Von Sachs
Julius Von Sachs showed that glucose is mainly stored as ______.
Starch
______ showed that green substances (chlorophyll) are located special bodies (chloroplasts ).
Von Sachs
What did Julius Von Sachs prove -
He found that GREEN PARTS is where GLUCOSE IS MADE and that the GLUCOSE is usually stored as STARCH .
______ used a prism and split light into its spectral components and then illuminated a green algae , (CLADOPHORA ) placed in a suspension of aerobic bacteria
T.W.Engelmann
The bacteria used in Engelmann’s experiment were used to detect sites of _____
O2 EVOLUTION
_____observed that bacteria accumulated mainly in the region of blue and red light of the split spectrum .
TW Engelmann
Who described the first ACTION SPECTRUM of photosynthesis ?
Engelmann
_____spectra of photosynthesis resembles _____ roughly the ____spectra of ________ .
Action
Absorption
Chloro a and b
What were Engelmann’s experiments and what did he observe?
Using a prism ,he split light into its spectral components and then illuminated green algae,CLADOPHORA placed in a suspension of AEROBIC bacteria . The bacteria were used to detect sited of O2 evolution .
He observed that bacteria accumulated in the region of blue and red light.
Empirical foramula of cabs -
CH2O
A milestone contribution to the understanding of photosynthesis was that made by a _____, ________.
Microbiologist
Cornelius van Neil
______ based o his studies on green and purple bacteria , demonstrated that photosynthesis is a light -dependent rxn .
Cornelius van Neil
Cornelius van Neil worked on _______ and ____ bacteria and demonstrated ______.
Purple green
Photosynthesis is a light-dependent rxn
Van Neil showed that H2 from a suitable _____ reduces ______ to ____ .
Oxidisable compound
CO2
Carbs
Write the expression based on van neil’s studies -
2 H2A + CO2 ———> 2A +CH2O+ H2O
In green plants _____ is the H donor and is oxidised to O2 .
H2O
Some organisms do not release O2 during photosynthesis .T/F
T
_____ is the H-donor for purple and green sulphur bacteria and the oxidation product is _____ or _____ depending on the organism and not O2 .
H2S
S or SULPHATE
Who inferred that O2 evolved comes from water and not form CO2 .
Cornelius van Neil
This was later proved by RADIOISOTOPIC tech
_____was proved using radioisotopic tech .
O2 is evolved from H2O
What did CORNELIUS van Neil prove ?
Photosynthesis is a light -dependent rxn in which H from a suitable oxidisable compound reduces CO2 to carbohydrates
_____cells in the leaves have a large number of chloroplasts .
MESOPHYLL cells
How to CHLOROPLASTS align themselves to get optimum quantity of incident light ?
Along the walls of the mesophyll cells
Within the chloroplasts , there is a _____ system consisting of grana, stroma lamella , and matrix stroma .
Membranous
The membranous system in chloroplasts consists of -
Grana
STROMA lamella
STROMA matrixa
There is a clear division of labour within the ___
Chloroplast
The membrane system in chloroplast is responsible for -
2 things-
Trapping light energy
Synthesis of ATP and NADPH
In _____ (part of membrane system ) , ENZYMATIC reactions synthesise SUGAR which in turn forms _____.
STROMA
Starch
Rxns like trapping light energy and synthesis of NADPH and ATP are _____.
Light dependent
Photochemical rxns
Dark reactions are also called -
Carbon rxns
We can separate the leaf pigments of any green plant through -
Paper chromatography
A chromatographic separation of the leaf pigments shows that the colour that we see in leaves is not due a a single pigment but due to _____ pigments .
4
What are the 4 main pigments of leaf -
Chloro a (bright or blue green ) Chloro b (yellow green ) Xanthophylls (yellow) Carotenoids (yellow to yellow orange )
______ are substances that have an ability to absorb light , at SPECIFIC WAVELENGTHS .
Pigments
Wavelengths at which there is max absorption of ______ (blue and red regions ) also shows higher rate of ______.
Chloro a
Photosynthesis
______ is the chief pigment associated with photosynthesis .
Chloro a
There is a complete one to one overlap between the ______ spectrum of chloro a and the ______ spectrum of photosynthesis.
Absorption
Action
Most of the photosynthesis takes place in the ___ and _____ regions of the spectrum .
Blue
Red
Some photosynthesis also takes place at other wavelengths of the visible spectrum (apart from blue and red ) .T/F
T
What are accessory pigments ? Name -
Chloro b
Xanthophyll
Carotenoids
Other thylakoids pigments that absorb light and transfer the energy to chloro a
______ enable a wider range of incoming light to be utilised for photosynthesis .
Accessory pigments .
_____prevent chloro a from photo-oxidation.
Accessory pigments
Light rxn or photochemical have includes __ , ____ and ___ .
light absorption
Water splitting
O2 release
Formation of high edgy chemical intermediates -ATP NADPH
The _____ are organised into ___ discreet photochemical _______ within the PS1 and PS2 .(light rxn)
Pigments
2
Light harvesting complexes (LHC)
The photosystems are named int he sequence of their _____.
Discovery
The ___ are made up of hundreds of pigment molecules bound to proteins .
LHC
Associated pigments protect chloro a from ________.
Photo-oxidation
Each photo system has all the pigments ( except chloro a ) forming a light harvesting system also called ______
Antennae
The pigments of photosystems forming an antennae help to make photosynthesis more EFFICIENT by -
Absorbing diff wavelengths of light
Which pigment in photosystems does not form a LHS or antennae -
One molecule of chloro a
The single chloro a molecule forms the ______ .
Reaction centre
The _____ is diff in both photosystems .
Reaction centre
In PS1 ,the reaction centre chloro a has an ABSORPTION peak at ______ ,hence is called _____ , while in PS2 it has absorption maxima at _______ , an is called _____
700nm
P700
680
P680
In photosystem ____ , the rxn centre chloro a absorbs 680nm of ___ light causing electrons to become excited and jump into an orbit farther from the atomic nucleus .
2
Red
The electron from PS 2 are picked up by an electron acceptor which passes them to an _____ consisting of _______.
Electrons transport system
Cytochromes
The ,movement of electrons through an ETS consisting on cytochromes is ___ in terms of ___ or __scale .
Downhill
Oxidation -reduction
Redox potential
The electrons from PS2 re not used up as they pass through ETS but are passed onto -
Pigments of PS1
The electrons in the rxn centre of PS1 also get excited when they receive __ light of wavelength ___.when does this happen ?
Red
700nm
When electrons from PS2 enter PS1
The excited e- from PS1 are transferred to another acceptor molecule that has ____.
Greater redox potential
The excited e- from PS1 move _____ , this time to a molecule of ___
Downhill
Energy-rich NADP+
What do the electrons from PS1 finally do ?
Reduce NADP+ to NADPH and H+
The whole scheme of transfer of e- starting from PS2 ___ to the acceptor down the ETC to PS1 , excitation of e- , transfer to another acceptor and finally downhill to NADP+ reducing it to NADPH + H+ is called __
Uphill
Z-scheme
The e- transfer scheme is called Z-scheme due to its __.
Characteristic shape
The Z shape of the scheme is formed when all the carriers are placed in a sequence on a ____.
Redox potential scale
Electrons from PS2 are transported _____ to the acceptor (up/downhill)
Uphill
How does PS22 supply e- continuously ?
E- removed from PS2 are replaced by e- available due to splitting of water .
The splitting of water is associated with PS __
2
Water is split into _ ,__ and _.
2H+
[O]
e-
The e- needed to replace those removed from PS1 are provided by -
PS2
Water s[plitting complex is associated with ps2 which is located on the ____ side of membrane of _____.
Inner
Thylakoid
Equation of water splitting -
2 H2O———> 4H+ +O2 +4e-
If ps2 is located on the inner side of membrane of thylakoid , then where are the protons and O2 likely to be released ?
Lumen of thylakoid
Living organism have the capability of extracting energy from ___substances and store this in the form of ____.
Oxidisable
Bond energy
ATP carry energy in their ___
Chemical bonds
ATP is synthesised by cells (in _ and __ ) is named _____.
Mitochondria
Chloroplast
Phosphorylation
______ is the synthesis of ATP from ADP and inorganic phosphate in the presence of light .
Photophosphorylation
When the 2 PS work in a series , a process called ________ occurs.
Non-cyclic photo-phosphorylation
The 2 PSs are connected through an -
ETC
What is synthesised at the end of non-cyclic photophosphorylation ?
ATP and NADPH + H+
Photophosphorylation is the synthesis of _____, from ___ and ____in the presence of light .
ATP
ADP
Inorganic phosphate
When only ____ is functional , the e- is circulated within the photosystem and cyclic phosphorylation occurs .
PS1
Cyclic phosphorylation occurs in the ______ of chloroplast .
STROMA lamella
The _______ has both PS1 and PS2 , therefore non-cyclic phosphorylation occurs here .
Membrane or lamella of grana
The _____ lack PS2 , therefore cyclic-phosphorylation occurs here.
STROMA lamella membrane
The STROMA lamella membrane lacks _ and _____ .
Both PS2 and NADP reductase enzyme .
Cyclic phos. Occurs here
In cyclic phosphorylation the excited e- does not pass on to ___ but are cycled back to ____ through the ETC
NADP+
PS1
The end products of cyclic phosphorylation .
Only ATP
_____ phosphorylation occurs when ONLY LIGHT of wavelengths BEYOND 680nm are available for excitation .
Cyclic
Cyclic phos occurs when only light of wavelengths ______ are available for excitation .
Beyond 680nm
The ___ hypothesis has been put forward to explain the mechanism of ATP synthesis in the chloroplast .
Chemiosmotic