Phlebotomy (Syringe Evacuated Method) Flashcards
Place tourniquet above the puncture site at
3-4 inches
Application time of tourniquet
less than 1 minute
Palpate _____
antecubital fossa area
Cleanse site with friction in a
back-and-forth motion
Let air dry
30-60 seconds
If blood cultures -
special iodine scrub procedure
If alcohol levels -
do not use alcohol to cleanse the site (use a non-alcohol-based disinfectant)
Hold syringe or vacutainer in
right hand; thumb on top, fingers underneath
Introduce needle into vein; bevel up at
15-30 degree angle
In ETS, release tourniquet when
when the last tube is 1/2 filled
Instruct the px to apply pressure to the area, preferably with arm held above head for _____
2-3 mins
If artery is accidentally struck, phlebotomist applies pressure for ____
10 minutes
Order of DRAW
Stop Light Green Light Go
SPS - Yellow
Coagulation tubes - Light Blue
Plastic clot activator/Serum separator tubes - Red
Heparin tubes - Green
EDTA - Lavender, Pink, Pearl top
Oxalate/Fluoride tubes - Gray
Minimizes chance of microbial contamination
Yellow SPS and Sterile media bottle
The first additive tube in the order because all other additives affect coagulation tests
Light blue
Filled after coagulation tests because silica particles activate clotting and affect coagulation tests (carryover of silica into subsequent tubes can be overridden by anticoagulant in them)
Red tube
Specimens: causes the least interference in tests other than coagulation tests
Green tube
Responsible for more carry-over problems than any other additive
Lavender
Role of EDTA (Lavender tube)
- Elevates Na+ and K+ levels
- Chelates and decreases calcium and iron levels
- Elevates PT and PTT results
Affects sodium and potassium levels, respectively, after hematology tubes because this damages cell membranes and causes abnormal RBC morphology
Gray tube (Sodium fluoride and Potassium oxalate)
Interferes in enzyme reactions
Gray (Oxalate
