Peripheral Blood Smear Staining: Wright's Stain Flashcards
Acidic Stain
Eosin
Basic Stain
Methylene BlueFi
Fixative
CH3OH (Methanol)
Buffer
0.5 M sodium phosphate or aged distilled water
pH of buffer
6.4-6.8
Stain for ___
3-10 mins
Buffer for ____
2-5 mins
Addition of buffer
Metallic sheen when blown
Water/drying artifact has several appearances:
- Moth-eaten look to RBCs
- Heavily demarcated central pallor
- Refractile blotches on the RBCs
- Sometimes manifests as echinocytes
Features of a well-stained peripheral blood film:
Macroscopically:
Should be pink to purple
Features of a well-stained peripheral blood film:
RBCs
Orange to salmon pink
Features of a well-stained peripheral blood film:
WBC nuclei
purple to blue
Features of a well-stained peripheral blood film:
neutrophil cytoplasm
pink to tan with violet or lilac granules
Features of a well-stained peripheral blood film:
Eosinophils granule:
bright orange refractile
Indication of abnormalities or test results that needs checking:
a film that is bluer overall
Plasma cell myeloma
Rouleaux
Indication of abnormalities or test results that needs checking:
Grainy appearance
RBC agglutination
Indication of abnormalities or test results that needs checking:
Holes
Increased lipid levels
Indication of abnormalities or test results that needs checking:
Blue specks our at the feather edges
Markedly increased WBC and platelet count
