PHAN LAB FINALS 1 Flashcards
Separations isolate the analyte from potentially
interfering constituents.
ANALYTICAL SEPARATION
What are the small-scale separation techniques
- Precipitation
- Distillation
- Extraction
- Ion-exchange
- Chromatography
It is a process in which a mixture of inert materials, drug principles, and impurities is separated into its components with different and reversible affinities
Chromatographic Method
A fluid that moves through or over the surface of the
stationary phase.
Mobile Phase
Mobile Phase is usually an _______________
Organic (Non-Polar) Solvent
It a fixed bed core of a large surface area
Stationary Phase
Stationary Phase is usually an __________
Adsorbent (Silica Gel G – a polar substance)
The simplest type of chromatographic column consists of a separation flask and a cylindrical glass tube constricted at one
end.
Column Chromatography
What are the phases in Column Chromatography
Mobile phase: organic solvent
Stationary phase: silica gel
Cellulose of the filter paper is used as adsorbent
Paper Chromatography (PC)
Phases in Paper Chromatography
Mobile phase: organic solvent (non-polar)
Stationary phase – water (polar)
It follows the same principle as the PC
o Most distinct result
o Most precise
TLC
Factors that affect the Rf Value
Nature adsorbent
Mobile phase
Thickness of layer
Temperature
Equilibrium
Dipping zone
Chromatographic techniques
It is used to enable the detection of separated compounds that are colorless and cannot be visualized with UV radiation or fluorescence.
Derivatization
What are the derivatization methods
Spraying
Dipping
In-situ derivatization
