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PHAN > Chromatography + > Flashcards

Chromatography + Flashcards

1
Q

It is a process in which a solution of mixture containing inert materials, drug principles and impurities is separated into its components

A

Chromatography

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2
Q

It brings about the sep1a1ration of a mixture through a competitive process in which the molecules of the mobile phase compete with the analyte

A

Adsorption Chromatography

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3
Q

The chromatographic process for adsorption

A

liquid-solid chromatography (LSC)

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4
Q

Chromatographic technique in which the mobile and
stationary phases are liquids referred to as liquid-liquid chromatography (LLC).

A

Partition Chromatography

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5
Q

Separation in partition elution chromatography is based upon what law?

A

Nernst’s law.

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6
Q

Law when two practically immiscible solvents are in contact with each other and substance which is soluble in each is added, the substance distributes itself in such a way that at equilibrium

A

Nernst’s law.

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7
Q

the ratio of the distance travelled over the paper
sheets by a given compound to the distance travelled
by the front of the mobile phase, from the point of
application of the test substances.

A

Retardation Factor(Rf)

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8
Q

Formula for Retardation Factor(Rf)

A

distance solute moves
Rf = ——————————
distance solvent moves

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9
Q

The ratio between the distances travelled by a
given compound and a reference substance.

A

RetardationReference (Rr)

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10
Q

Formula for retardation reference

A

distance solute moves
Rr = ———————————————–
distance reference standard moves

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11
Q

The materials used to pack column for use in Ion-
exchange chromatography are either _____________

A

cationic or anionic

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12
Q

The stationary phase consists of a polymeric matrix onto the surface of which ionic functional groups, The mobile phase used in this chromatography are always liquid.

A

ion-exchange chromatography

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13
Q

This is also known as gel filtration or gel permeation chromatography,

A

Molecular Exclusion Chromatography

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14
Q

The simplest type of chromatographic column
consists of a separation flask and a cylindrical glass
tube constricted at one end.

A

Column Chromatography

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15
Q

When the compounds are radioactive, their position
on the column may be determined by the use of a __________ counter

A

Geiger-Muller counter.

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16
Q

The basic principle of separation in the partition
chromatography is that of difference in partition
coefficients of substances between two immiscible
liquids.

A

Paper-Partition Chromatography

17
Q

Chromatography that is accomplished by allowing the mobile phase to flow downward on the
paper strip.

A

Descending Chromatography

18
Q

Chromatography in which the mobile phase
is allowed to rise upward on the paper by capillary
attraction

A

Ascending Chromatography

19
Q

Chromatography in which the mobile phase
moves out in concentric circles from the center of a
circular piece of paper.

A

Radial Chromatography

20
Q

Chromatography that involves the spotting of a sampler of a mixture of components at one end of an adsorbent-coated glass plate

A

Thin-Layer Chromatography

21
Q

TLC Achieves separations in a relatively short time of
about ____________ or less using as little as ________ ng of material

A

30 minutes
20 ng of material

22
Q

they are the most widely used adsorbents.

A

Silica Gel G
Alumina

23
Q

Chromatography that is based on partition phenomenon but the non- polar solvent is fixed to the paper or solid column material. especially useful in the separation of water-insoluble substances such as steroids.

A

Reversed-Phase Chromatography

24
Q

method of separation based on different migration
of the solute between the stationary phase and the
mobile phase.

A

Gas Chromatography

25
Q

It defined as the time required by an average
molecule of component to pass from the injection
point through the column to the detector.

A

Retention time (tR)

26
Q

Unit used in Retention time (tR)

A

(minutes per millimeters)

27
Q

Defined as the volume of carrier gas necessary to
carry an average molecule of the component from
the point of injection to the detector.

A

Retention Volume (vR)

PHAN (11 decks)

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