Chromatography + Flashcards
It is a process in which a solution of mixture containing inert materials, drug principles and impurities is separated into its components
Chromatography
It brings about the sep1a1ration of a mixture through a competitive process in which the molecules of the mobile phase compete with the analyte
Adsorption Chromatography
The chromatographic process for adsorption
liquid-solid chromatography (LSC)
Chromatographic technique in which the mobile and
stationary phases are liquids referred to as liquid-liquid chromatography (LLC).
Partition Chromatography
Separation in partition elution chromatography is based upon what law?
Nernst’s law.
Law when two practically immiscible solvents are in contact with each other and substance which is soluble in each is added, the substance distributes itself in such a way that at equilibrium
Nernst’s law.
the ratio of the distance travelled over the paper
sheets by a given compound to the distance travelled
by the front of the mobile phase, from the point of
application of the test substances.
Retardation Factor(Rf)
Formula for Retardation Factor(Rf)
distance solute moves
Rf = ——————————
distance solvent moves
The ratio between the distances travelled by a
given compound and a reference substance.
RetardationReference (Rr)
Formula for retardation reference
distance solute moves
Rr = ———————————————–
distance reference standard moves
The materials used to pack column for use in Ion-
exchange chromatography are either _____________
cationic or anionic
The stationary phase consists of a polymeric matrix onto the surface of which ionic functional groups, The mobile phase used in this chromatography are always liquid.
ion-exchange chromatography
This is also known as gel filtration or gel permeation chromatography,
Molecular Exclusion Chromatography
The simplest type of chromatographic column
consists of a separation flask and a cylindrical glass
tube constricted at one end.
Column Chromatography
When the compounds are radioactive, their position
on the column may be determined by the use of a __________ counter
Geiger-Muller counter.
The basic principle of separation in the partition
chromatography is that of difference in partition
coefficients of substances between two immiscible
liquids.
Paper-Partition Chromatography
Chromatography that is accomplished by allowing the mobile phase to flow downward on the
paper strip.
Descending Chromatography
Chromatography in which the mobile phase
is allowed to rise upward on the paper by capillary
attraction
Ascending Chromatography
Chromatography in which the mobile phase
moves out in concentric circles from the center of a
circular piece of paper.
Radial Chromatography
Chromatography that involves the spotting of a sampler of a mixture of components at one end of an adsorbent-coated glass plate
Thin-Layer Chromatography
TLC Achieves separations in a relatively short time of
about ____________ or less using as little as ________ ng of material
30 minutes
20 ng of material
they are the most widely used adsorbents.
Silica Gel G
Alumina
Chromatography that is based on partition phenomenon but the non- polar solvent is fixed to the paper or solid column material. especially useful in the separation of water-insoluble substances such as steroids.
Reversed-Phase Chromatography
method of separation based on different migration
of the solute between the stationary phase and the
mobile phase.
Gas Chromatography
