Perfection

Question 1

Gram stain

Answer 1

Bacteria and fungi

Question 2

Giemsa stains:

Answer 2

1. Acanthodea
2. Fungi
3. cytology
4. intracellular inclusion bodies

example: GEMMA BEGLEY PASSES TLYER CHYLAMYDIA

Question 3

ZIehl Neelson

Answer 3

Acid fast bacteria Mycobacterium and nocardia

Question 4

Calcoflour white:

Answer 4

Fungi and acanthamoeba

Question 5

KOH

Answer 5

Fungi

Question 6

Congo red stain ?

Answer 6

stains amyloid orange used in Lattice corneal dystrophy

Question 7

Trichrome stain?

Answer 7

Granular
Hyaline stained red by trichome

Question 8

Alcain blue

Answer 8

Stains mucopolysaccharide in macular dystrophy

Question 9

Von kossa

Answer 9

Stains calcuim black in band kerathopathy

Question 10

Eosin

Answer 10

Detects cytoplasmic organells stain pink

Question 11

Period acidic-schiff stains

Answer 11

stains basement membrane magenta of lens capsule, bruchs, descements

Question 12

Masson Trichrome?

Answer 12

Stains collagen blue/green
Stains hyaline red

Question 13

Luxol fat blue ?

Answer 13

Stains myelin blue - disappear when demyelinated

Question 14

Bodian stains:

Answer 14

Nerve fibres blakc

Question 15

Movats Pentacous:

Answer 15

elastic fibres black

Question 16

Muscarine

Answer 16

stains mucus pink

Question 17

wilders

Answer 17

Stains reticulum black

Question 18

haematoxylin and eosin

Answer 18

Nucleic acid within the nuclei stains blue

Question 19

India ink

Answer 19

India ink
Cryptococcus neoformans
stain is not taken up by polysaccharide capsule, creating a transparent halo
mucicarmine can also be used to stain capsule red

Question 20

Silver stain

Answer 20

Silver stain
fungi
e.g. Pneumocystis, Legionella

Question 21

Theory of gram staining

Answer 21

Gram-positive bacteria have a thick peptidoglycan layer in their cell walls that holds the crystal violet stain
Gram-negative bacteria cannot hold the crystal violet stain and are counterstained by safranin