PCV

Question 1

Stages-

Answer 1

put on gloves- PPE
fill EDTA two heamocrit tubes with blood from EDTA blood (3/4s)
wipe off any remaining residue- prevents contamination with surface/putty
keep finger on end- bung end 2/3 times, store on tray
REPEAT

place both in centrifuge, putty facing outwards
tubes level- maintains balance
secure lid
dispose of PPE and equipment appropriately

Question 2

Equipment Needed-

Answer 2

heamocrit tubes x 2
gloves
tissue
putty/ buffer
EDTA blood

Question 3

Legislation-

Answer 3

HASAWA (1974)
COSHH (2002)
HWR (2005)

Question 4

How to read-

Answer 4

select hawksley
placed tube sealed end downwards
align bottom of RBC layer
move holder until lined up with 100% line
move adjustable pcv reading line- matching top of RBC layer
record PCV %
dispose