PCR

Question 1

Polymerase Chain rxn

Answer 1

technique to make many copies of specific region of DNA

Question 2

Tac polymerase

Answer 2

used to denature template DNA/separate the strands
heat tolerant/stable
most active at 70degrees C

Question 3

DNA polymerase

Answer 3

used to make new strands of DNA

Question 4

PCR primers

Answer 4

short sequence of nucleotides that provides a starting point for DNA synthesis
single-stranded DNA, usually around
20 nucleotides in length

Question 5

Step 1 -Denature

Answer 5

96°C
Heat the reaction strongly to separate, or denature, the DNA strands
provides single-stranded template

Question 6

Step 2-Anneal

Answer 6

55 - 65°C

Cool the reaction so the primers can bind to their complementary sequences on the single-stranded template DNA

Question 7

Step 3 -Extend

Answer 7

72°C

Raise the reaction temperatures so Taq polymerase extends the primers, synthesizing new strands of DNA

Question 8

short tandem repeats (STRs)

Answer 8

consist of many repeating copies of the same short nucleotide sequence (typically,
22 to 55 nucleotides long)

Question 9

polymorphic

Answer 9

many alleles that vary in tiny increments of length

Question 10

How are the results of PCR visualized?

Answer 10

Gel electrophoresis
fragments of DNA are pulled through a gel matrix by an electric current, and it separates DNA fragments according to size
can be compared with a DNA ladder