Gel Electrophoresis Flashcards
1
Q
Step 1
A
Protein sample denatured using SDS (neg charge)
heats binds SDS to pr- =unfolding
2
Q
Step 2 -if pr- has many subunits
A
SDS also dissociates into individual polypeptide chains
3
Q
Step 3
A
Sample loaded into well with polyacrylamide gel
4
Q
What electrode does the sample travel to?
A
negative sample travels to positive electrode at bottom of well
5
Q
How does size affect movement through gel?
A
smaller fragments travel farther/quicker than larger
less MW has high mobility
6
Q
Gel electrophoresis
A
separates fragments by size
used to visualize PCR results
