Pathophysiology of Inflammation Flashcards
What is the purpose of inflammation?
- Mobilize and attract immune components to the site
- Neutralize invasion of microbes and limit their spread
- Remove debris and prepare for repair
- Finally, RESOLVE when the job is done
What are the two types of inflammation?
Acute and Chronic
Describe acute inflammation
- Short in duration (<2 weeks)
- Involves discrete set of events
- Localized to the area of injury or infection
- Critical for human health
Describe chronic inflammation
- More diffuse area
- Extends over longer period
- Usually maladaptive/harmful
- May result in scar tissue formation, tissue destruction, deformity
- May be the cause of illness and disability
Inflammation Path Summary (Based on the diagram)
Injury
Neutrophils + macrophages // Vasoactive chemicals // Chemokines
Vasodilation + Vascular permeability
Emigration of neutrophils and macrophages into tissue
Phagocytosis
Acute Inflammation - Sequence of Events
- Precipitating Event: tissue damage or microbial invasion
- Activation of local inflammatory cells (macrophages, dendritic and mast cells, neutrophils)
- Generation of local mediators: leukotrienes, prostaglandins, bradykinin, histamine, complement cascade, neutrophil proteases, ROS. Causes VASODILATION, VASCULAR PERMEABILITY
- Recruitment and emigration of more neutrophils, macrophages, plasma proteins, including clotting proteins (if needed)
- Bacteria, debris, dead cells phagocytosed
- If wound present, platelets seal the area –> clotting
- Resolution of inflammation: healing, fibrosis, scar
How does inflammation lead to edema?
Increased vascular permeability of blood vessels
The gaps between endothelial cells are widened, allowing plasma proteins and leukocytes to enter the site of tissue damage
Fluid leakage through those vessels results in EDEMA
What are the cardinal signs of inflammation?
- Pain
- Heat
- Redness
- Swelling
(loss of function could be a fifth!)
Signs and Symptoms of Inflammation
- Fever
- Neutrophilia (release of neutrophils)
- Lethargy, feeling tired
- Muscle catabolism (increase AA pool for antibody production)
- Liver releases acute phase proteins in response to IL-1, IL-6, TNF-alpha (pro-inflammatory cytokines released primarily by macrophages, can be measured in patient’s blood)
What are acute-phase proteins?
A protein whose plasma concentrations increase during inflammation
Innate Immunity
Does not require previous exposure to antigen
Rapid action, quick resolution
Kill invading organisms via phagocytic cells, NK, protein cascade
Acquired Immunity
Adaptive Immunity
Previous exposure required
Slower initial activation, can last a lifetime (but response can be rapid on second exposure)
Immune cells (T cells, B cells)
Cell-mediated immunity (cytotoxic killer T cells)
Humoral immunity (B cells, antibodies)
What are the two pathways for leukocyte (WBC) development?
Lymphoid
Myeloid
What does the lymphoid pathway give rise to?
Lymphocytes
NK cells
What does the myeloid pathway give rise to?
Granulocytes, monocytes, macrophages, dendritic cells, megakaryocytes, erythrocytes
What do Helper T cells do?
- Activate other T cells and macrophages
- Stimulate B cell proliferation and antibody production
- Secrete pro-inflammatory cytokines
What do cytotoxic, or “killer” T cells do?
Kill infected cells (NOT the pathogen, the pathogen-infected cells)
What do B lymphocytes do?
Created and developed in bone marrow
Produce antibodies
Long-lived
Have memory cells that produce daughter cells programmed to produced the same antibodies
Differentiate into plasma cells, “antibody factories” that can synthesize large quantities of antibodies when they are needed
What is the relationship between T helper cells and B cells?
T helper cells help activate b cells
Antibodies are produced by _____
B lymphocytes
Are soluble form of BCRs, circulate in blood, ly,ph, found in mucus
Describe an antibody’s structure
Four polypeptide chains
two long heavy chains
two short light chains held together by disulfide bonds
What are some antibody functions?
- Function as antitoxins, bind and neutralize bacterial toxins
- Are OPSONINS: Coat antigens and make it more recognizable to phagocytes
- Can activate complement cascade (when bound to antigens on cell membranes)
- Form antibody-antigen complexes that precipitate out of body fluids, problematic in certain autoimmune disorders
Neutrophils, eosinophils, mast cells, and basophils are examples of _____
Granulocytes - Myeloid lineage
Where are mast cells found?
Found in tissues, especially tissues that interface the external environment
Where are basophils found?
Found in the circulation
Which cells are a part of the myeloid lineage?
Granulocytes (neutrophils, eosinophils, mast cells, basophils)
Antigen-presenting cells (monocytes, macrophages, dendritic cells)
RBCs, megakaryocytes
What are granulocytes?
White blood cells that have cytoplasmic granules containing enzymes needed to kill bacteria and other invaders
How do granulocytes work (intracellularly and extracellularly)?
Intracellularly - break down pathogens that are engulfed and endocytosed
Extracellularly - they release into the nearby environment by the granulocyte, can cause tissue damage
Which cell is the primary cell type of acute inflammation?
Neutrophils
They arrive quickly to the site
Function of neutrophils
Move from blood into tissue
Phagocytose and kill invading organisms, then undergo apoptosis
How do neutrophils arrive to the area of injury?
Chemotaxis // chemotactic factors
includes complement fragments and cytokines