Parts of the Microscope Flashcards
What is the eyepiece or ocular in a microscope, and what is its magnification?
The eyepiece (ocular) is the lens through which the object is viewed, and it magnifies the image by 10x. It also has a pointer to pinpoint parts of the object.
What is the function of the revolving nosepiece in a microscope?
The revolving nosepiece holds four objective lenses and can be rotated to switch between them. It clicks when each lens is in proper position for viewing.
What does the objective lens do in a microscope?
The objective lens provides the initial magnification and is located on the revolving nosepiece. Lower magnifications have shorter objective lenses that are positioned farther from the specimen when focused.
What is the magnification and purpose of the scanning objective lens?
The scanning objective is the shortest lens and magnifies the object 4x. It provides the broadest field of view and is used for initial focusing.
What is the magnification of the low-power objective (LPO)?
The low-power objective (LPO) magnifies by 10x.
What is the magnification of the high-power objective (HPO), and how is it different from the LPO?
The high-power objective (HPO) magnifies by 40x and is much longer than the LPO.
What is the oil immersion objective (OIO) and its magnification?
The oil immersion objective (OIO) is the longest objective lens and magnifies by 100x.
What is the stage of a microscope, and what does it do?
The stage is the flat surface with a round aperture (gap/opening) where the slide is positioned. It can have a mechanical device or stage clips to hold the slide.
What is the function of the substage condenser in a microscope?
The substage condenser, located under the stage aperture, concentrates light from the source and focuses it on the specimen.
What does the iris diaphragm do in a microscope?
The iris diaphragm controls the amount of light passing through the specimen and can be adjusted to change light intensity.
What is the substage illuminator?
The substage illuminator is the light source that illuminates the object being viewed. It can sometimes be replaced by a mirror.
What is the function of the illuminator control knob?
The illuminator control knob, located on the base or below the mechanical stage knobs, adjusts the light output of the illuminator.
What are the adjustment knobs on a microscope, and how are they used?
The adjustment knobs control focusing. The coarse adjustment knob (larger) is used with scanning and low-power objectives, while the fine adjustment knob is used with high-power and oil-immersion objectives.
