Parts of Microscope Flashcards

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1
Q

Ocular Lens (eyepiece)

A

Magnify object

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2
Q

Body Tube

A

Holds eyepiece and objective lenses from each other

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3
Q

Revolving Nosepiece

A

Rotating disk that holds 2+ objective lenses

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4
Q

Objective lens(es)

A

Magnify object (every lens has different power

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5
Q

Arm

A

Connects base and tube.

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6
Q

Stage

A

Supports microscope slide

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7
Q

Stage Clips

A

Hold slide in position. Allows light to pass through in the center

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8
Q

Light source

A

Source that illuminates the specimen

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9
Q

Condenser Lens

A

Directs light to object being viewed

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10
Q

Diaphragm

A

Controls amount of light reaching object being viewed

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11
Q

Coarse Adjustment Knob

A

Moves tube vertically to bring object to focus

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12
Q

Fine Adjustment Knob

A

Brings object into sharper focus

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13
Q

Types of Objective Lens

A

Low Power [4X]
Medium Power [10X]
High Power [40X]

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14
Q

Calculate Total Magnification

A

Total Magnification = Ocular Lens X Objective Lens

Example: 10X • 4X = 40X

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15
Q

Diameter Field of View (dFOV)

A

Diameter of what you see through the lens in a microscope

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16
Q

Calculate dFOV

A

Transparent ruler on stage and measure under LOW power

17
Q

Relationship between dPOV and details

A

As dPOV decreases,
Detail increases
From low to high power

18
Q

Magnification equation

A

dFOV (1) x total magnification (1) = dFOV (2) x total magnification (2)

19
Q

Calculate object size

A

Object size = dPOV(μm)/number of times object fits across dFOV