Parasitology 2 Flashcards

1
Q

What is a sediment test used for?

A

Parasites with high density that wont float readily (fluke and other unusual parasites)

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2
Q

How do you perform a sediment test?

A

Mix feces with water, allow it to settle, fluid poured off leaving sediment, repeat

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3
Q

What is the Baermann test?

A

Easy, used for infections where larvae are the diagnostic stage - need a fresh sample with live larvae, allow it to sit 8-24hr at room temp and larvae migrate into water

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4
Q

Is one negative baermann test a definitive negative?

A

NO

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5
Q

So you see larvae in the baremann test.. now what?

A

Kill them with Lugol’s iodine or heat (visualize)

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6
Q

If you perform the baermanns test and see nematodes of the same size with tail with kink and spine what are they most likely?

A

Aelurostrongylus (Lung worm)

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7
Q

What if you perform baermanns test and the parasites are roughly the same size, but have a plain tail?

A

Free living nematode or hatched hookworm

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8
Q

What is the bulb?

A

Muscle at end of esophagus that pumps to move food though the intestines

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9
Q

If you find larva with a kink tail in dogs on a baermann test then you should suspect…

A

Spurious parasite - aelurostongylus eating cat poo, mulleris eating small ruminant or camelid poop, or deer poop

Only K9 would be angiostronglus

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10
Q

Can you detect parasites in urine?

A

Yes, with sedimentation you can recover eggs passed in urine (free catch may be contaminated)

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11
Q

What stain should be used to investigate parasites on blood smear?

A

Wrights or Giemsa

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12
Q

What parasites can you see on blood smear?

A

Hemoprotozoa, intracellular and extracellular (Babesia, theileria, cytauxzoon and trypanosoma)

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13
Q

When does taking a bloodsmear work best?

A

Acute infection

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14
Q

What can you see on a direct unstained blood smear?

A

Microflora or nonpathogenic

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15
Q

What is a good way to get a concentrated blood exam?

A

Knott Test

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16
Q

What can you collect from skin for morphologic exam?

A

Hair, feathers, skin scruf, organisms, skin scrape

17
Q

How do you do a skin test?

A

Make a slide with a drop of oil, coverrslip, nail polish around exterior

18
Q

If submitting samples what should you use to preserve samples of worms?

A

70-90% ethanol (preserve DNA)

19
Q

What are the most common helminths in small animals?

A

Ascarids (toxocara) and Tapeworms

20
Q

What are some immunologic tests that can be performed? What does a positive on these antibody tests mean?

A

Elisa, Ifa and other
Mean exposure and immune response

21
Q

What are some commonly used antibody tests in small animal?

A

Dirofilaria in cats, babesia, protozoa (toxo, neospora, trypanosoma, leishmania)

22
Q

What is an antigen test?

A

Look for piece of parasite
Elisa or IFA
-Dirofilariasis immitis
-Giardia

23
Q

When should you test for giardia?

A

If there is diarrhea, not screening

24
Q

When testing for giradia what is gold standard?

A

Antigen and zinc sulfate