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Question 1

FIXATIVES FOR SMEARS

Answer 1

1. SCHAUDINN’S
2. ETHER-ALCOHOL
3. METHANOL

Question 2

FIXATIVES FOR SCHAUDINN’S

Answer 2

➢ Mercuric Chloride
➢ Absolute Ethanol
➢ Acetic acid

Question 3

OTHER METHODS OF FIXATION

Answer 3

1. Heat

2. Microwave Fixation

Question 4

METHODS FOR MICROWAVE FIXATION

Answer 4

1. VACUUM
2. HEAT AND AGITATION (10-15 MM)
3. FORMALDEHYDE FIXATIVES

Question 5

CHEMICAL FIXATION IS AVOIDED

Answer 5

SPECIAL TECHNIQUES

Question 6

Recommended for histochemical studies

Answer 6

SPECIAL TECHNIQUES

Question 7

Rapid freezing of tissue blocks to allow instant cessation of cellular activities

Answer 7

QUENCHING

Question 8

FREEZING AGENTS

Answer 8

1. Liquid Nitrogen
2. Isopentane
3. Pentane
4. Propane
5. Dichlorodifluoromethane

Question 9

METHODS FOR FREEZE-DRYING

Answer 9

1. RAPID FREEZING

2. DESICCATION

Question 10

tissue plunged in isopentane or propane-isopentane

Answer 10

RAPID FREEZING

Question 11

TEMPERATURE FOR RAPID FREEZING

Answer 11

(-160 to -180 OC)

Question 12

removal of tiny ice crystals by sublimation in a vacuum

Answer 12

DESICCATION

Question 13

TEMPERATURE FOR DESICCATION

Answer 13

(-30 to -40 OC)

Question 14

Similar with freeze-drying

Answer 14

FREEZE SUBSTITUTION

Question 15

Instead of dehydration in a vacuum, tissue blocks IN FREEZE SUBSTITUTION are:

Answer 15

✓ Fixed in Rossman’s fluid or 1% acetone

✓ Dehydrated with abs. alcohol