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FIXATION > PAGE 4 > Flashcards

PAGE 4 Flashcards

1
Q

for routine paraffin sections, electron microscopy, histochemistry and enzyme studies

A

ALDEHYDE FIXATIVES

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2
Q

gas produced by the oxidation of methyl alcohol

A

FORMALDEHYDE (FORMALIN)

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3
Q

BUFFERED PH OF FORMALDEHYDE (FORMALIN)

A

7 TO 8

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4
Q

leads to acidity which favors the formation of Formalin heme pigments

A

HYPOXIA IN TISSUES

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5
Q

OTHER TERM FOR FORMALDEHYDE

A

FORMALIN

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6
Q

black, polarizable deposits

A

FORMALIN HEME PIGMENTS

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7
Q

PURE STOCK OF FORMALDEHYDE

A

40% FORMALIN

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8
Q

DILUTION OF FORMALDEHYDE FOR 10% SOLUTION

A

1:10

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9
Q

DILUTION OF FORMALDEHYDE FOR 5% SOLUTION

A

1:20

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10
Q

CONCENTRATION OF FORMALDEHYDE FOR FIXATION

A

10%

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11
Q

white precipitate due to prolonged storage

A

PARAFORMALDEHYDE

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12
Q

REMOVAL OF PARAFORMALDEHYDE

A
  1. FILTRATION

2. ADDITION OF 10% METHANOL

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13
Q

ALDEHYDE FIXATIVES

A
  1. FORMALDEHYDE (FORMALIN)
  2. 10% FORMOL-SALINE
  3. 10% NEUTRAL BUFFERED FORMALIN
  4. FORMOL-CORROSIVE
  5. ALCOHOLIC FORMALIN
  6. GLUTARALDEHYDE
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14
Q

central nervous tissues and general post-mortem tissues for histochemical examination

A

10% FORMOL SALINE

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15
Q

ideal with most stains including silver impregnation

A

10% FORMOL SALINE

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16
Q

DURATION OF FIXATION OF 10% FORMOL SALINE

A

more than 24 hours (slow fixative)

17
Q

preservation and storage of surgical, post-mortem and research specimens

A

10% NEUTRAL BUFFERED FORMALIN

18
Q

TYPES OF FIXATIVE

A
  1. ALDEHYDE FIXATIVE
  2. METALLIC FIXATIVE
  3. PICRIC ACID FIXATIVE
  4. GLACIAL ACETIC ACID
  5. ALCOHOL FIXATIVE
  6. OSMIUM TETROXIDE
  7. TRICHLOROACETIC ACID
  8. ACETONE

FIXATION (13 decks)

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