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PAGE 3 Flashcards

1
Q

contain glacial HAc

A

NUCLEAR FIXATIVE

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2
Q

high affinity to nuclear chromatin

A

Glacial HAc

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3
Q

destroys mitochondria and Golgi bodies

A

Glacial HAc

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4
Q

pH OF NUCLEAR FIXATIVE

A

4.6 OR LESS

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5
Q

preserve cytoplasmic structures

A

CYTOPLASMIC FIXATIVES

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6
Q

do not contain glacial HAc

A

CYTOPLASMIC FIXATIVE

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7
Q

pH OF CYTOPLASMIC FIXATIVE

A

MORE THAN 4.6

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8
Q

preserve chemical constituents

A

HISTOCHEMICAL FIXATIVES

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9
Q

HISTOCHEMICAL FIXATIVE FOR LIPIDS

A

mercuric chloride or potassium dichromate

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10
Q

HISTOCHEMICAL FIXATIVE FOR PHOSPHOLIPIDS

A

Baker’s formol-calcium

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11
Q

HISTOCHEMICAL FIXATIVE FOR CHOLESTEROL

A

digitonin

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12
Q

HISTOCHEMICAL FIXATIVES FOR CARBOHYDRATES

A

alcoholic fixatives

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13
Q

HISTOCHEMICAL FIXATIVE FOR GLYCOGEN

A

Rossman’s fluid or cold absolute alcohol

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14
Q

HISTOCHEMICAL FIXATIVE FOR PROTEINS

A

neutral buffered formol saline or formaldehyde vapor

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15
Q

HISTOCHEMICAL FIXATIVE FOR ELECTRON MICROSCOPY

A

double fixation

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16
Q

MIXTURE FOR ELECTRON CYTOCHEMISTRY

A

Karnovsky’s paraformaldehydeglutaraldehyde solution

17
Q

MIXTURE FOR ACROLEIN

A

mixture of glutaraldehyde or fomaldehyde

18
Q

rapid penetration, preserves morphology and enzyme activity at low concentrations

19
Q

immersion fixation of surgical biopsies

20
Q

FIXATIVES FOR MICROANATOMICAL

A
  • 10% Formol saline
  • 10% Neutral buffered formalin
  • Heidenhain’s SuSa
  • Formol sublimate/corrosive
  • Zenker’s solution
  • Zenker-formol/Helly’s solution
  • Bouin’s solution
  • Brasil’s solution
21
Q

FIXATIVES FOR CYTOPLASMIC

A
  • Helly’s
  • Orth’s
  • Regaud’s/Muller’s
  • Flemming’s
  • Formalin with post-chroming
22
Q

FIXATIVES FOR NUCLEAR

A
  • Heidenhain’s SuSa
  • Newcomer’s
  • Bouin’s
  • Flemming’s
  • Carnoy’s
23
Q

FIXATIVES FOR HISTOCHEMICAL

A
  • 10% Formol saline
  • Absolute ethanol
  • Newcomer’s
  • Acetone
24
Q

CHEMICAL CONSTITUENTS FOR HISTOCHEMICAL FIXATIVE

A
  1. LIPIDS
  2. PHOSPHOLIPIDS
  3. CHOLESTEROL
  4. CARBOHYDRATES
  5. GLYCOGEN
  6. PROTEINS
  7. ELECTRON MICROSCOPY

FIXATION (13 decks)

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