PACT: Osmolality and Electrophoresis

Question 1

What analyze could be responsible for an osmolal gap of 40 most/kg

Answer 1

ethanol

Question 2

What action is required for a turbid sample prior to performing an osmolality measurement by freezing point depression

Answer 2

centrifuge the sample to remove particles

Question 3

What would be the potential effect on serum osmolality results if residual alcohol remained after performing cleaning maintenance

Answer 3

falsely elevated

Question 4

T or F: and elevated glucose result will produce an increased osmolal gap

Answer 4

false

Question 5

In SPE, what is the outcome when protein is placed in a buffer with a pH greater than the isoelectric point of the protein?

Answer 5

an anion that migrated towards the anode

Question 6

In SPE, what would cause all of the protein bands to be clustered together near the point of application?

Answer 6

using a buffer with a neutral pH

Question 7

In SPE, which of the following would cause protein migration to appear faster than normal

Answer 7

using a buffer with a higher pH

Question 8

In SPE, what would be the effect of using a buffer of low ionic strength

Answer 8

fast migration of proteins

Question 9

In SPE, what would be the outcome of using a plasma sample instead of serum

Answer 9

additional dark staining at the point of application