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Mol Bio Lab > (P) L2: Reagent Preparation > Flashcards

(P) L2: Reagent Preparation Flashcards

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1
Q

T or F: Most reagents come in their diluted form

A

False (concentrated)

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2
Q

This piece of equipment weighs out desired amounts of chemicals such as powder or liquid reagents especially if it’s less than 0.1 grams

A

Analytical Balance

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3
Q

T or F: Use regular paper when measuring powder reagents

A

False (baking or wax paper)

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4
Q

You place chemicals into appropriate-sized beakers using this device to guide the liquid

A

Stir Bar

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5
Q

When using this piece of equipment to dissolve solutes, add less than the required volume solvent first before adding the other half

A

Graduated Cylinder

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6
Q

If you are using water, what is the appropriate decontaminated form to use in a laboratory?

A

Double-distilled

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7
Q

This sterilizes the solution, reagents, and equipment

A

Autoclave

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8
Q

Give the appropriate parameters for the autoclave

A

Temperature: 121ºC
Duration: 20-30 minutes
Pressure: 15 psi

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9
Q

This traps the unsterile particles to give way for the sterile solution; make sure the paper is single use only—used for only small amounts of liquid

A

Filtration

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10
Q

What is the paper measurement for filter sterilization?

A

0.22 or 0.45 µm

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11
Q

A standard but essential instrument that precisely and accurately transfers volumes of liquid within small microliters in volume

A

Micropipette

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12
Q

What is the difference between a single-channeled and a multi-channeled micropipette?

A

Single-channeled only aspirates one volume while multi-channeled has multiple pipette tips

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13
Q

What is more commonly used in the laboratory, single or multi-channeled micropipette?

A

Single

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14
Q

T or F: The single-channeled micropipette reduces workload compared to the multi-channeled

A

False (used for large volume of samples)

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15
Q

What kind of micropipettes are used where it aspirates a certain amount of liquid and dispenses almost the exact amount of air?

A

Air displacement micropipettors

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16
Q

Parts of a micropipette:
> it is connected to the plunger and helps in measurement

A

Piston and cylinder system

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17
Q

Parts of a micropipette:
> pushes the air volume equal to the liquid to be aspirated

A

Plunger

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18
Q

T or F: The plunger adjusts the volume by turning up or down to decrease or increase the volume level

A

False (clockwise and counterclockwise)

19
Q

Parts of a micropipette:
> some pipettes have this same function with their plunger

A

Volume adjustment knob

20
Q

Parts of a micropipette:
> it pushes the ejector arm to remove the pipette tips to prevent contact upon disposal

A

Tip ejector

21
Q

Parts of a micropipette:
> it offers fitting for the pipette tips

A

Tip cone

22
Q

T or F: The pipette should be held in a vertical position

A

True

23
Q

What is the appropriate immersion depth in forward pipetting?

A

1-3mm

24
Q

What is the appropriate immersion depth for REVERSE pipetting?

*note: it has it’s own depth acc. to the ppt

A

2-5mm below the solution surface

25
Q

What happens when you immerse the pipette too deeply?

A

Potential hole blockage

26
Q

What happens when you immerse the pipette too shallow?

A

Potential air bubbles

27
Q

How much should the container be tilting diagonally?

A

10º-40º

28
Q

A P20 pipette can hold what volume range?

A

2-20

29
Q

A P200 pipette can hold what volume range?

A

20-200

30
Q

A P1000 pipette can hold what volume range?

A

200-1000

31
Q

A P5000 pipette can hold what volume range?

A

1000-5000

32
Q

What is the tip color for a P20 pipette?

A

Yellow

33
Q

What is the tip color for a P200 pipette?

A

Yellow

34
Q

What is the tip color for a P1000 pipette?

A

Blue

35
Q

What is the tip color for a P5000 pipette?

A

White

36
Q

T or F: It is better to choose the smallest pipette capable of handling your desired volume

A

True

37
Q

Forward or Reverse Pipetting?
> used more commonly for liquid samples

A

Forward

38
Q

Forward or Reverse Pipetting?
> used more frequently for viscous samples such as oil and blood

A

Reverse

39
Q

When do you press until the second stop for forward pipetting?

A

When dispensing

40
Q

When do you press until the second stop for reverse pipetting?

A

Before aspiration

41
Q

T or F: You can clean the micropipette tip orifice using tissues

A

False (the tissue can possibly absorb the liquid contents inside the pipette making the volume not exact anymore)

42
Q

T or F: Set the volume of the micropipette to the maximum capacity before use

A

False (after)

43
Q

T or F: Always use a new tip for each new substance

A

True

Mol Bio Lab (12 decks)

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