L1: Basic Laboratory Practices (MANUAL-BASED)

Question 1

Practice of precautionary measure to minimize possible exposure of the laboratory worker to hazards coming from materials that are potentilaly infectious

Answer 1

Laboratory Biosafety

Question 2

Is the application of practices that will protect, regulate, and account for microorganisms held in the laboratory that will avert their misuse, loss, illicit access, pilfering, or deliberate release.

Answer 2

Laboratory Biosecurity

Question 3

T or F

Biosecurity is imperative because handling of infectious microorganisms poses potential hazards to the laboratory personnel and the environment

Answer 3

F (BIOSAFETY IS IMPERATIVE)

Question 4

In this biosafety level, the microorganisms handled are usually non-pathogenic in healthy individuals and pose negligible risk to the laboratory worker and the environment

Answer 4

Biosafety level 1

Question 5

Escherichia coli and other non pathogenic disease are example of what biosafety level?

Answer 5

Biosafety level 1

Question 6

In this biosafety level, microorganisms usually moderately hazardous to the lab personnel and the environment

The microbes usually infect humans and produce diseases of different degrees of severity.

They include microorganisms that cause mild symptoms to human or are not usually acquired through airborne particles in a laboratory environment.

Answer 6

Biosafety level 2

Question 7

Examples of this biosafety level are Staphylococcus aureus, Chlamydiae, Hepatitis A, B, and C, influenza A, Salmonella, Mumps, and Measles.

(CHIMMSS)

Answer 7

Biosafety level 2

Question 8

In this biosafety level, laboratory personnel work on microorganisms that are either indigenous or exotic.

These microbes are known to cause serious or potentially deadly disease through transmission by inhalation

Answer 8

Biosafety level 3

Question 9

Examples of this biosafety level are Mycobacterium tuberculosis, Covid-19 virus, HIV, H1N1, Yersinia pestis, SARS, Rabies, West Nile viruses, and Rickettsia

Answer 9

Biosafety level 3

Question 10

In this biosafety level, The microorganisms handled are dangerous and unusual.

There is a high risk of transmission through aerosol. T

The infections caused by the microorganisms usually cause lethal infections, and there are no available treatment and vaccine.

Answer 10

Biosafety level 4

Question 11

Examples of this biosafety level are Ebola and Marburg virus

Answer 11

Biosafety level 4

Question 12

T or F

If the skin or face is accidentally splattered by corrosive or harmful chemicals, bacterial or cell culture, immediately go to clinic

Answer 12

F (USE EMERGENCY SAFETY SHOWER AND FLUSH SKIN WITH WATER FOR ATLEAST 15 MINUTES)

Question 13

If eyes are splashed with chemicals, what should you do?

Answer 13

use eyewash station

Question 14

For injury with contaminated sharp items (broken glassware, syringe, etc.) what are the appropriate steps to do?

Answer 14

1. Wash with water
2. Consult clinic

Question 15

What do you do when a large volume of highly hazardous chemical occurs?

Answer 15

EVACUATE the AREA (wag mo nang ayusin yung hindi na maayos)

Question 16

Spillage of large volumes (>1L) of cell culture is cleaned up with?

Answer 16

Paper towels and decontaminated with 10% bleach and 70% ethyl alcohol

Question 17

What percentage of bleach and ethyl alcohol do you use when there is spillage of more than 1 liters of cellculture=

Answer 17

10% bleach
70% ethyl alcohol

Question 18

Any waste that contains contagious substances or possibly
infectious materials such as blood

Answer 18

Biohazard waste

Question 19

Sharps waste are to be put in what after use?

Answer 19

Shaprs disposal box/puncture proof containers

Question 20

T o F

Immediately throw broken glass into containers

Answer 20

F (autoclave first)

Question 21

Gloves used for work with biohazard material are disposed in?

Answer 21

solid biohazard waste

Question 22

How are used agar or any solid material disposed?

Answer 22

Autoclaved at 121°C for 30 min under 15 psi

Question 23

How are cultures disposed?

Answer 23

disinfected with 10% bleach solution for 30 min or autoclaved at 121°C for 30 min at 15 psi, and subsequently discarded on the sink

Question 24

How are reusable flasks and bottles disposed?

Answer 24

autoclaved or rinsed with 10% bleach/sodium hypochlorite

Question 25

T or F

Small amount (<100 mL) of acids or bases may be neutralized and slowly poured in the sink with lots of running water.

Answer 25

T

Question 26

T or F

Mix acids and bases when disposing of chemical waste

Answer 26

F (DO NOT MIX THEM)

Question 27

T or F

Most buffers can be poured down the sink.

Answer 27

T

Question 28

T or F

Hazardous chemical waste such as solvents should be flushed down the sink.

Answer 28

F (Hazardous chemical waste such as solvents should NOT be flushed down the sink)