L1: Basic Laboratory Practices (MANUAL-BASED) Flashcards
Practice of precautionary measure to minimize possible exposure of the laboratory worker to hazards coming from materials that are potentilaly infectious
Laboratory Biosafety
Is the application of practices that will protect, regulate, and account for microorganisms held in the laboratory that will avert their misuse, loss, illicit access, pilfering, or deliberate release.
Laboratory Biosecurity
T or F
Biosecurity is imperative because handling of infectious microorganisms poses potential hazards to the laboratory personnel and the environment
F (BIOSAFETY IS IMPERATIVE)
In this biosafety level, the microorganisms handled are usually non-pathogenic in healthy individuals and pose negligible risk to the laboratory worker and the environment
Biosafety level 1
Escherichia coli and other non pathogenic disease are example of what biosafety level?
Biosafety level 1
In this biosafety level, microorganisms usually moderately hazardous to the lab personnel and the environment
The microbes usually infect humans and produce diseases of different degrees of severity.
They include microorganisms that cause mild symptoms to human or are not usually acquired through airborne particles in a laboratory environment.
Biosafety level 2
Examples of this biosafety level are Staphylococcus aureus, Chlamydiae, Hepatitis A, B, and C, influenza A, Salmonella, Mumps, and Measles.
(CHIMMSS)
Biosafety level 2
In this biosafety level, laboratory personnel work on microorganisms that are either indigenous or exotic.
These microbes are known to cause serious or potentially deadly disease through transmission by inhalation
Biosafety level 3
Examples of this biosafety level are Mycobacterium tuberculosis, Covid-19 virus, HIV, H1N1, Yersinia pestis, SARS, Rabies, West Nile viruses, and Rickettsia
Biosafety level 3
In this biosafety level, The microorganisms handled are dangerous and unusual.
There is a high risk of transmission through aerosol. T
The infections caused by the microorganisms usually cause lethal infections, and there are no available treatment and vaccine.
Biosafety level 4
Examples of this biosafety level are Ebola and Marburg virus
Biosafety level 4
T or F
If the skin or face is accidentally splattered by corrosive or harmful chemicals, bacterial or cell culture, immediately go to clinic
F (USE EMERGENCY SAFETY SHOWER AND FLUSH SKIN WITH WATER FOR ATLEAST 15 MINUTES)
If eyes are splashed with chemicals, what should you do?
use eyewash station
For injury with contaminated sharp items (broken glassware, syringe, etc.) what are the appropriate steps to do?
- Wash with water
- Consult clinic
What do you do when a large volume of highly hazardous chemical occurs?
EVACUATE the AREA (wag mo nang ayusin yung hindi na maayos)
Spillage of large volumes (>1L) of cell culture is cleaned up with?
Paper towels and decontaminated with 10% bleach and 70% ethyl alcohol
What percentage of bleach and ethyl alcohol do you use when there is spillage of more than 1 liters of cellculture=
10% bleach
70% ethyl alcohol
Any waste that contains contagious substances or possibly
infectious materials such as blood
Biohazard waste
Sharps waste are to be put in what after use?
Shaprs disposal box/puncture proof containers
T o F
Immediately throw broken glass into containers
F (autoclave first)
Gloves used for work with biohazard material are disposed in?
solid biohazard waste
How are used agar or any solid material disposed?
Autoclaved at 121°C for 30 min under 15 psi
How are cultures disposed?
disinfected with 10% bleach solution for 30 min or autoclaved at 121°C for 30 min at 15 psi, and subsequently discarded on the sink
How are reusable flasks and bottles disposed?
autoclaved or rinsed with 10% bleach/sodium hypochlorite
T or F
Small amount (<100 mL) of acids or bases may be neutralized and slowly poured in the sink with lots of running water.
T
T or F
Mix acids and bases when disposing of chemical waste
F (DO NOT MIX THEM)
T or F
Most buffers can be poured down the sink.
T
T or F
Hazardous chemical waste such as solvents should be flushed down the sink.
F (Hazardous chemical waste such as solvents should NOT be flushed down the sink)
