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Mol Bio Lab > (P) L1: Basic Lab Practices > Flashcards

(P) L1: Basic Lab Practices Flashcards

1
Q

This protects you and your clothing from contamination, should not be worn outside the lab

A

lab coats

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2
Q

What type of footwear is appropriate for lab use?

A

closed shoes to protect the foot from punctures, and corrosive / infectious materials

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3
Q

When should gloves be changed?

A

when contaminated

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4
Q

these (2) equipment are worn when you are about to be in contact with hazardous aerosols and caustic chemicals

A

eyewear and masks

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5
Q

order of proper donning of PPE

A

gown, mask, goggles, and gloves

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6
Q

order of proper doffing of PPE

A

gloves, goggles, gown, mask

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7
Q

when should you wash your hands

A

before and after completion of work

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8
Q

T or F

Storing of food in the laboratory is fine as long as it is not in contact with the reagents

A

F

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9
Q

T or F

Applying cosmetics in the lab is fine as long as you wash your hands first

A

F

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10
Q

Where are sharps disposed of?

A

puncture-proof containers

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11
Q

T or F

Needles should be recapped from the syringes

A

F

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12
Q

This minimizes the creation of aerosols / splashes

A

closed containers

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13
Q

Where should infectious materials should be manipulated

A

biosafety cabinet

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14
Q

Which chemicals may be used to decontaminate work sufaces?

A

10% bleach or 70% ethyl alcohol

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15
Q

What steps (2) should be done before disposing infectious materials

A

placed in a biohazard bag or autoclaved

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16
Q

Where should incidents be reported?

A

appropriate personnel

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17
Q

Use this card to familiarize steps to do in a biological accident / emergency

A
  1. use safety showers for 15 minutes if exposed to any corrosive chemicals and bacterial culture splatters; use the eyewash if necessary
  2. wash with water and consult with the clinic if injured by contaminated sharp items (e.g. broken glassware, syringes, etc.)
  3. use paper towels in cleaning spillage of more than 1L of cell cultures (paper towels are to be autoclaved); decontaminate with 10% bleach or 70% ethyl alcohol
  4. evacuate if there is spillage of large volumes of hazardous chemicals
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18
Q

studies the structure and function of molecules and macromolecular systems associated with biological processes

A

molecular biology

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19
Q

Molecular biology focuses on the study of _____ and _______

A

nucleic acids and proteins

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20
Q

Molecular biology isolates and identifies _____ / ______ materials from different samples

A

genetic / protein

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21
Q

What are the typical methods employed in molecular biology?

A

PCR, gel electrophoresis, protein analysis, and sequencing techniques

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22
Q

T or F (Lab design)

Physical facilities of COVID testing shall have adequate and appropriate areas to provide service to the clients

A

T

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23
Q

T or F (Lab design)

Bidirectional workflow shall be maintained at all times

A

F (Unidirectional) str8 tayo d2

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24
Q

T or F. The airflow of a reagent prep room/area should be positive pressure.

A

T

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25
Q

What is the direction of positive pressure

A

outward

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26
Q

What is the direction of negative pressure?

A

inward

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27
Q

2 reasons for separate areas

A
  1. Introduction of unwanted nucleic acids may lead to contamination
  2. The sensitivity of molecular techniques makes them vulnerable to contamination
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28
Q

3 ways to control contamination

A

laboratory design, lab practices, and chemical and enzymatic controls

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29
Q

use this card to familiarize the potential sources of contamination

A
  1. cross contamination between specimens
  2. amplification of product contamination
  3. lab surfaces
  4. ventilation ducts
  5. reagents / supplies
  6. hair, skin, saliva, and clothes
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30
Q

a physical method of sterilization by killing bacteria, viruses, and spores

A

autoclave

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31
Q

How do autoclaves kill bacteria, viruses, and spores

A

it uses steam under pressure (heat)

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32
Q

What are the different levels of decontamination

A
  1. cleaning
  2. disinfecting
  3. sterilization
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33
Q

level of decontamination where bacteria is minimized

A

cleaning

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34
Q

level of decontamination where viable organisms are eliminated

A

disinfecting

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35
Q

level of decontamination where viable organisms and their spores are eliminated

A

sterilization

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36
Q

equipment that protects the workers, environment, and materials from infectious or biohazardous aerosols and splashes

A

Biological safety cabinet (BSC)

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37
Q

what are the three classes of BSC?

A

class I, II, and III

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38
Q

BSC characterized to have an open front with directional airflow that protects the personnel and environment using a HEPA filtered exhaust and operates under negative pressure

A

Class I

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39
Q

What is the biosafety level requirement of containment for class I BSC

A

1-3

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40
Q

What BSC class is characterized with an open front with directional airflow for personnel and environmental protection with HEPA-filtered laminar down flow for product protection

A

Class Ii

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41
Q

How many HEPA filter(s) is/are in a class I BSC

A

1

42
Q

How many HEPA filter(s) is/are in a class II BSC

A

2 (HEPA exhaust and HEPA filter laminar downflow)

43
Q

How many HEPA filter(s) is/are in a class III BSC

A

3

44
Q

What are the different types of class II BSC

A

A1, A2, B1, B2

45
Q

What is the most common type of Class II BSC

A

A2 (90%)

46
Q

Why is type B Type II BSC not popular?

A

requires very specific installation and operating conditions

47
Q

Which risk groups does Type II BSC work with (given that positive pressure suits are used)

A

2,3, and 4

48
Q

where is the air discharged in type II BSC

A

rear plenum and into the space between supply and exhaust filters

49
Q

how much air pass through the exhaust HEPA filters in class II BSC

A

30%

50
Q

How much air is recirculated through the HEPA filter in class II BSC

A

70%

51
Q

This class of BSC cabinet provides the highest level of protection

A

Class III

52
Q

T or F

Class III biosafety cabinets has no holes at all and utilizes automated machines to handle infectious specimens

A

F there are holes sealed with gloves

53
Q

Fume hood or BSC?

Filters and blowers are incorporated

A

BSC

54
Q

Fume hood or BSC?

Protects the user only

A

Fume hood

55
Q

Enumerate what the safety cabinet protects

A

personnel, product, environment (PPE)

56
Q

Where does the vapors and aerosols go when using a fume hood

A

outside the building

57
Q

T or F

Safety cabinets does not exhaust outside the building without decontamination

A

T

58
Q

BSC types for personnel protection, microorganisms in risk group 1-3

A

Class I, II, and II

59
Q

BSC type for personnel protection, microorganisms in risk group 4, glove box lab

A

class III

60
Q

BSC type for personnel protection, microorganism in risk group 4, suit laboratory

A

Class I, Class II

61
Q

BSC type for product protection

A

Class II, Class III (only if laminar flow included)

62
Q

BSC type for volatile radionuclide / chemical protection, minute amounts

A

Class IIB1, Class IIA2 vented to the outside

63
Q

BSC type for radionuclide / chemical protection

A

Class I, Class IIB2, Class III

64
Q

T or F

Chemical fume hoods must be near the BSC

A

F (must not be near)

65
Q

T or F

BSC must be near anything that created air movement for better ventilation

A

F (must not be near)

66
Q

T or F

BSC must be far from the entrance, near the rear of the laboratory, away from traffic

A

T

67
Q

T or F

BSCs are certified at initial installation and quarterly thereafter

A

F (annually thereafter)

68
Q

T or F

BSCs must be certified after moving, replacement of filters, and maintenance

A

T

69
Q

T or F

BSCs must be certified according to the manufacturer’s instructions

A

t

70
Q

Use this card to familiarize yourself with the utilization of BSCs

A
  1. Magnahelic gauge should be checked regularly
  2. The airflow pattern should not be disrupted
  3. Disinfect the interior and materials
  4. Should run at least 5 minutes after the work volume is set up
  5. Open flames are to be avoided
  6. Decontamination
71
Q

These are made from pleated borosilicate glass arranged in random fibers

A

HEPA filters

72
Q

what does HEPA stand for

A

High efficiency particulate air

73
Q

fill in the blanks

HEPA filters traps _____% of particles larger than _____ in diameter

A

99.97% of particles larger than 0.3 micrometer

74
Q

This produces an amplicon that may be used for gel electrophoresis

A

conventional PCR

75
Q

this checks the concentration and purity of sample

also measures how much a chemical substance absorbs light by measuring its intensity as a beam is passed through a sample solution

A

spectrophotometer

76
Q

this mixes small vials of liquid

A

vortex mixer

77
Q

this separates components of liquids that have different weights

A

centrifuge

78
Q

this delivers an accurately measured volume of solution

A

automatic pipettors

79
Q

what are the most frequently used type of pipettors

A

micropipettes

80
Q

micropipette range measures are from ______ to less than _______

A

1000µl (1mL) to less than 1µl

81
Q

T or F. The airflow of a sample preparation room/area should be positive pressure.

A

F (negative)

82
Q

T or F. The airflow of a amplification room/area should be positive pressure.

A

F (negative)

83
Q

In establishing a molecular testing in Clinical lab environments the workflow of rooms should be in what sequence?

A

Reagent prep room -> sample prep room -> ampli room

84
Q

designed to maintain the pressure differential between the lab and its adjacent space; not all rooms have the same pressure.

A

ante room

85
Q

T or F. The appropriate BSC for cell & tissue culture, propagation of virus is BSC 1.

A

F

86
Q

This BSC is commonly found in clinical and research laboratories working with infectious agents or with tissue culture.

A

BSC 2

87
Q

The class that is associated with BSL 1, 2, and 3 containments

A

BSC 2

88
Q

air circulated and exhausted: A1 & A2

A

70% recirculated and 30% exhausted

89
Q

air circulated and exhausted: B1

A

70% exhausted and 30% recirculated

90
Q

air circulated and exhausted: B2

A

100% exhaust

91
Q

Which of the following does not belong: BSC Class II air flow

A. Once room air enters, instead of directly proceeding to the work surface, it will first go to the open/front exhaust grill,
B. Then it will go down, then up, then some air
will be exhausted and some air will be recirculated
C. And then the air in the work surfaces will go
to the front or rear exhaust grill.
D. None of the above.

A

D

92
Q

What BSC Class has a double HEPA filter exhaust, autoclave and dumptank for disposal.

A

3

93
Q

What BSC class is suitable for work in Biosafety Level 3 and 4 laboratories.

A

3

94
Q

The HEPA filter traps particles of what diameter?

A

0.3 micrometer in diameter

95
Q

T or F. Anything larger to 0.3 can be efficiently filtered by HEPA filter such as vapor and gases.

A

F (gases and vapors r relatively smaller)

96
Q

used to amplify a specific region of any DNA sample; the target is amplified

A

thermocycler

97
Q

a type of PCR that uses gel electrophoresis and UV transilluminator

A

conventional

98
Q

A microcentrifuge is designed for small tubes between ml?

A

0.2mL and 2mL

99
Q

ideal ref temp

A

-20c and -40c

100
Q
A

Mol Bio Lab (12 decks)

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