L4: Reagent Preparation (MANUAL-BASED)

Question 1

Use this card to familiarize the steps in reagent preparation

Answer 1

1. Measure the required weight of each component using an analytical balance.
2. Place the chemicals into a beaker with a stir bar.
3. Add double distilled water with a volume 20% less than the required total amount.
   Dissolve the reagents thoroughly, if available, use a magnetic stirrer.
4. If the pH of the solution needs to be adjusted, do so first using a pH meter. Calibrate the
   pH electrode first using buffers with known pH.
5. Transfer the dissolved reagent to a graduated cylinder. Add the remaining required
   volume of water until the final volume of solution is achieved.
6. When preparing solutions with agar or agarose powder, weigh out the powder directly into the final container such as an Erlenmeyer flask.
7. Autoclave the solutions whenever necessary.
8. To avoid contamination of stock solutions, prepare working solutions by diluting the stock solutions with sterile double distilled water.

Question 2

Reagent Prep:

Measure the required weight of each component using an ?

Answer 2

Analytical balance

Question 3

Place the chemicals into a beaker with?

Answer 3

stir bar

Question 4

T or F

Add double distilled water with a volume 20% less than the required total amount.

Answer 4

T

Question 5

T or F

Solvent added should be more than required total amount

Answer 5

F (LESS THAN)

Question 6

If the pH of the solution needs to be adjusted, do so first using a?

Answer 6

pH meter

Question 7

Transfer the dissolved reagent to?

Answer 7

Graduated cylinder

Question 8

T or F

You add remaining volume of water after transferring into beaker

Answer 8

F (you add remaining volume after transferring GRADUATED CYLINDER)

Question 9

When preparing solutions with agar or agarose powder, weigh out the powder directly into the final container which is?

Answer 9

Erlenmeyer flask

Question 10

T or F

Prepared reagents should be autoclaved

Answer 10

T

Question 11

To avoid contamination of stock solutions, prepare working solutions by?

Answer 11

Diluting stock solutions with sterile double distilled water