L2: Laboratory Equipment (MANUAL-BASED)

Question 1

Is a machine that is used to sterilize materials that are resistant to heat such as biological waste, culture media, instruments, and laboratory ware.

Answer 1

Autoclave

Question 2

Autoclave uses a combination of _____, _____, ______

Answer 2

steam, pressure, time

Question 3

A special device used to determine the acidity or alkalinity of a
substance

Answer 3

pH meter

Question 4

T or F

The unit of measure expressing the level of acidity or alkalinity of a substance is pH

Answer 4

T

Question 5

A procedure that separates components of liquids that have different weight

Answer 5

Centrifuge

Question 6

What should you increase for complete and rapid sedimentation of precipitate to the bottom of vessel?

Answer 6

Revolution per minute (rpm) or G force (gravitational force)

Question 7

Are designed for small tubes of between 0.2 mL and 2.0 mL.

Answer 7

Microcentrifuge

Question 8

T or F

When using a centrifuge, it is important to always balance the tubes before being run

Answer 8

T

Question 9

T or F

Tubes in microcentrifuge must have equal weight

Answer 9

T

Question 10

What do you do when tubes do not have identical components or unequal volume?

Answer 10

Either weigh tubes and make them equal

OR

use another tube of the same capacity nd fill solution with equal volume

Question 11

T or F

Always place the tubes with the hinge facing the inner rim of the rotor

Answer 11

F (Always place the tubes with the hinge facing the OUTER rim of the rotor)

Question 12

Are used for samples that require a constant range of temperature.

Answer 12

Refrigerated centrifuge

Question 13

Temperature range of refrigerated centrifuge

Answer 13

between -20 and -40 degrees c

Question 14

T or F

Refrigerated centrifuge speeds of less than 20,000 rpm with relative centrifugal force of greater than 65,000 x g

Answer 14

F (speeds of MORE THAN 30,000 rpm with relative centrifugal force of greater than 65,000 x g)

Question 15

an instrument used to measure how much a chemical substance absorbs light by mesuring internsity of light as beam of light passes through solution

Answer 15

Spectrophotometer

Question 16

A device is used to amplify (like a photocopier) a specific region
of any DNA sample with polymerase chain reaction (PCR) in a test tube

Answer 16

Thermocycler

Question 17

T or F

In a thermocycler, The sample is first denatured by heating—forming two single-stranded DNA

Answer 17

T

Question 18

In thermocycler, it copies original DNA forming what?

Answer 18

New molecule consiting of one old and new DNA strand

Question 19

T or F

In thermocycling, amplification is repeated in about 20 to 25 cycle

Answer 19

F (repeated in about 30 to 40 cycle)

Question 20

an apparatus used for viewing DNA or
protein molecules separated by agarose or polyacrylamide gel electrophoresis.

Answer 20

UV Transilluminator

Question 21

The rate of migration in gel electrophoresis depends upon its?

Answer 21

net charge, size, shape, electric current

Question 22

Micropipettes measure volumes that range from?

Answer 22

1000 ul (1ml) to less than 1 ul

Question 23

playful conversation starts

Answer 23

counter all your quick remarks like passing notes in secrecy