Oxidative Phosphorylation Flashcards
How many protons are pumped out of the matrix for each pair of electrons that enter the ETC?
10
What two complexes is ATP synthase constructed from
transmembrane F0 sub-complex and F1 knobs
Where is the F1 complex located?
on the matrix side of the inner mitochondrial membrane
How many units does the c-ring comprise of?
8-15 subunits
What is the symmetry of the delta, gamma, epsilon subunits?
C1 symmetry
What amino acid is protonated to pass through the C ring?
glutamate/aspartate
What amino acid rips a proton off the glutamate residue?
arginine
How many degrees is one full rotation of the gamma subunit?
360
How many ATPs does one full rotation of the gamma subunit produce?
3 ATP
Which is the catalytic subunits in the a/B subunits?
B subunit
How many degress for the gamma subunit to change the conformation of the aB dimers?
120 degrees
Oxygen consumption is __ linked to substrate availability.
stoichiometrically
What does oligomycin do? How does it do this?
Inhibit ATP synthase. It binds to the glutamate residue in the c-ring subunits preventing H+ from flowing back to the matrix
Why does blocking ATP synthesis shut down the ETC?
Because the proton gradient becomes too steep, its highly thermodynamically unfavorable
The energetic need of the cell is in direct proportion to the cell’s response. What is this referred to as?
respiratory control
